ID A0A1G5C7B5_9FIRM Unreviewed; 433 AA.
AC A0A1G5C7B5;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000256|HAMAP-Rule:MF_01074};
DE Short=P2TMN nickel insertion protein {ECO:0000256|HAMAP-Rule:MF_01074};
DE EC=4.99.1.12 {ECO:0000256|HAMAP-Rule:MF_01074};
DE AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000256|HAMAP-Rule:MF_01074};
GN Name=larC {ECO:0000256|HAMAP-Rule:MF_01074};
GN ORFNames=SAMN02910371_00652 {ECO:0000313|EMBL:SCX98292.1};
OS Butyrivibrio sp. INlla14.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae;
OC Butyrivibrio.
OX NCBI_TaxID=1520808 {ECO:0000313|EMBL:SCX98292.1, ECO:0000313|Proteomes:UP000199551};
RN [1] {ECO:0000313|Proteomes:UP000199551}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=INlla14 {ECO:0000313|Proteomes:UP000199551};
RA Varghese N., Submissions S.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC (P2TMN), to form the mature cofactor. Is thus probably required for the
CC activation of nickel-pincer cofactor-dependent enzymes.
CC {ECO:0000256|HAMAP-Rule:MF_01074}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_01074};
CC -!- SIMILARITY: Belongs to the LarC family. {ECO:0000256|HAMAP-
CC Rule:MF_01074}.
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DR EMBL; FMVS01000004; SCX98292.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G5C7B5; -.
DR OrthoDB; 9765625at2; -.
DR Proteomes; UP000199551; Unassembled WGS sequence.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.70.1380; Transcriptional regulatory protein pf0864 domain like; 1.
DR HAMAP; MF_01074; LarC; 1.
DR InterPro; IPR002822; Ni_insertion.
DR NCBIfam; TIGR00299; nickel pincer cofactor biosynthesis protein LarC; 1.
DR PANTHER; PTHR36566; NICKEL INSERTION PROTEIN-RELATED; 1.
DR PANTHER; PTHR36566:SF1; NICKEL INSERTION PROTEIN-RELATED; 1.
DR Pfam; PF01969; Ni_insertion; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_01074};
KW Nickel {ECO:0000256|ARBA:ARBA00022596, ECO:0000256|HAMAP-Rule:MF_01074};
KW Reference proteome {ECO:0000313|Proteomes:UP000199551}.
FT REGION 80..110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 433 AA; 48305 MW; 0C02EA5CC4A99416 CRC64;
MKTLYIECKM GVAGDMLGAA LLDLLDEQEE VLAELNGIGI KGVEFKLTDT EKCGIKSKHL
RVLINGQEEI PEYIASDEGL DDHEHHDHEH SHEHDNGHHY HGHEDNGTLH EYDDIHEHDH
IHRTLYDIED IIEAMTVSKD IKSDVREIYE LLAEAESQAH GVPVDKIHFH EVGNLDAIAD
ITASCYLIHK LEPEKIIVSP INVGGGMVKT AHGVLPVPAP ATATLLKGIP SYESEYVRSE
LCTPTGAALI KYFADEFKPQ PLMAVEKIGY GAGNKDFAQA NVMRAVLGET TDEIENVIEL
ACNIDDMTAE EIGFATEMLF EAGALEVYTI AADMKKNRPG TLLYCVCKLD KRDLIVQTIF
KHTTTLGIRE NICNRYVLSR EIQKIDTPYG EVRKKVSSGY NVSRFKLEYD DIAGIAKRTG
KSILDLKKEL EEC
//
