ID   A0A1G5C912_9FIRM        Unreviewed;       238 AA.
AC   A0A1G5C912;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 19.
DE   SubName: Full=Pyruvate formate lyase activating enzyme {ECO:0000313|EMBL:SCX98821.1};
GN   ORFNames=SAMN02910371_00680 {ECO:0000313|EMBL:SCX98821.1};
OS   Butyrivibrio sp. INlla14.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae;
OC   Butyrivibrio.
OX   NCBI_TaxID=1520808 {ECO:0000313|EMBL:SCX98821.1, ECO:0000313|Proteomes:UP000199551};
RN   [1] {ECO:0000313|Proteomes:UP000199551}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=INlla14 {ECO:0000313|Proteomes:UP000199551};
RA   Varghese N., Submissions S.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR   EMBL; FMVS01000004; SCX98821.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1G5C912; -.
DR   OrthoDB; 9782387at2; -.
DR   Proteomes; UP000199551; Unassembled WGS sequence.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd01335; Radical_SAM; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR012840; NrdG2.
DR   InterPro; IPR034457; Organic_radical-activating.
DR   InterPro; IPR007197; rSAM.
DR   NCBIfam; TIGR02495; NrdG2; 1.
DR   PANTHER; PTHR30352:SF23; PYRUVATE FORMATE-LYASE 1-ACTIVATING ENZYME; 1.
DR   PANTHER; PTHR30352; PYRUVATE FORMATE-LYASE-ACTIVATING ENZYME; 1.
DR   Pfam; PF13353; Fer4_12; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01094; Uncharacterised_Radical_SAM_Su; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   4: Predicted;
KW   4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Lyase {ECO:0000313|EMBL:SCX98821.1};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Pyruvate {ECO:0000313|EMBL:SCX98821.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000199551};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT   DOMAIN          13..238
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000259|PROSITE:PS51918"
SQ   SEQUENCE   238 AA;  26953 MW;  56F6A5464181D188 CRC64;
     MQIHGLQKMT LLDFPGNVAC TIFLGGCDFR CPYCHNFELV DGSAEPIMDE GELFSFLEKR
     KGVLDGVCIT GGEPCLRQDL PDFIRRIKAL GYKIKLDTNG AHPDMVKELM DAQLIDYVAM
     DIKNCPEKYA ITAGLTGRAN SNISKHFNIE SVEQTASLLL EGRIDYEFRT TVVERYHTEA
     DFHEIGKWIT GAKKYFLQQY VPRDTVPDCT LTAPSRETME NFIKIVRQYV PTAELRGL
//