ID A0A1G5CFC2_9PROT Unreviewed; 843 AA.
AC A0A1G5CFC2;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=DNA ligase (ATP) {ECO:0000256|ARBA:ARBA00012727};
DE EC=6.5.1.1 {ECO:0000256|ARBA:ARBA00012727};
DE AltName: Full=NHEJ DNA polymerase {ECO:0000256|ARBA:ARBA00029943};
GN ORFNames=SAMN05216420_1028 {ECO:0000313|EMBL:SCY01179.1};
OS Nitrosospira sp. Nl5.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
OC Nitrosomonadaceae; Nitrosospira.
OX NCBI_TaxID=200120 {ECO:0000313|EMBL:SCY01179.1, ECO:0000313|Proteomes:UP000198645};
RN [1] {ECO:0000313|Proteomes:UP000198645}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nl5 {ECO:0000313|Proteomes:UP000198645};
RA Varghese N., Submissions S.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP +
CC diphosphate.; EC=6.5.1.1; Evidence={ECO:0000256|ARBA:ARBA00034003};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
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DR EMBL; FMVQ01000002; SCY01179.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G5CFC2; -.
DR STRING; 200120.SAMN05216420_1028; -.
DR OrthoDB; 9802472at2; -.
DR Proteomes; UP000198645; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-EC.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR CDD; cd07906; Adenylation_DNA_ligase_LigD_LigC; 1.
DR CDD; cd07971; OBF_DNA_ligase_LigD; 1.
DR CDD; cd04862; PaeLigD_Pol_like; 1.
DR Gene3D; 3.30.1490.70; -; 1.
DR Gene3D; 3.30.470.30; DNA ligase/mRNA capping enzyme; 1.
DR Gene3D; 3.90.920.10; DNA primase, PRIM domain; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR InterPro; IPR014146; LigD_ligase_dom.
DR InterPro; IPR014144; LigD_PE_domain.
DR InterPro; IPR014145; LigD_pol_dom.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR014143; NHEJ_ligase_prk.
DR InterPro; IPR033651; PaeLigD_Pol-like.
DR NCBIfam; TIGR02777; LigD_PE_dom; 1.
DR NCBIfam; TIGR02778; ligD_pol; 1.
DR NCBIfam; TIGR02779; NHEJ_ligase_lig; 1.
DR NCBIfam; TIGR02776; NHEJ_ligase_prk; 1.
DR PANTHER; PTHR42705; BIFUNCTIONAL NON-HOMOLOGOUS END JOINING PROTEIN LIGD; 1.
DR PANTHER; PTHR42705:SF2; MULTIFUNCTIONAL NON-HOMOLOGOUS END JOINING DNA REPAIR PROTEIN LIGD; 1.
DR Pfam; PF04679; DNA_ligase_A_C; 1.
DR Pfam; PF01068; DNA_ligase_A_M; 1.
DR Pfam; PF13298; LigD_N; 1.
DR Pfam; PF21686; LigD_Prim-Pol; 1.
DR SUPFAM; SSF56091; DNA ligase/mRNA capping enzyme, catalytic domain; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:SCY01179.1};
KW Manganese {ECO:0000256|ARBA:ARBA00023211};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000198645};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 317..423
FT /note="ATP-dependent DNA ligase family profile"
FT /evidence="ECO:0000259|PROSITE:PS50160"
SQ SEQUENCE 843 AA; 94975 MW; 16EC5ED752CC7DAA CRC64;
MAKPDLLKAY RAKRNFSITS EPSGIAKRGK STGAVLSFVV QKHWATRLHY DFRLELDGTM
KSWAVPKGPS FDPHDKRMAV HVEDHPISYN RFEGQIPTGQ YGAGKVIVWD KGTWIPLEDP
HKGYREGKLK FELRGHKLRG HWALVRMKNT EEKKKEPWLL IKEKDEYVRP ASEYSVVDEM
PDSVAKLESE GISKRAALVN DVRPSKKKLS GLPSGAAEAD LPRIMQPQLA TLVDALPNQP
ADWIYEIKFD GYRILARIDG RKIQLFTRNG NDWSHKLPDL VKAIGRMRLR PGWLDGEIVV
PNENGIPDFQ ALQNAFDNSR TQNIVYYLFD IPFYDGHDLR AAPLTERREF LRTLFKTPPP
EQVRFSEAFD APARDIVASV CRMGLEGVIG KRKSATYVSR RSPDWIKLKC SQRQEFVIGG
YTDPQGSRIG IGSLLLGFYD DDQKLRYAGN VGTGFSDKTL HELKAKLDKV AAIHSPFINA
SGIGKKAHWV RPTLVAEVSF GEWTRDGHIR HSVFHGLRTD KKAELIRREK PVHPSDINPS
RMTSIPSSLR VTHPERVIDP SSGFTKMDLI RYYSLVAPLM LEHIKGRPVS LVRAPDGIEG
QLFFQKHWEK ENMPGVAQLD PALDPEHEPL IEISTAEGLL SAAQMNVVEI HTWNATKNAI
GKPDRMTFDL DPGEGVQWAA MQESAQLVRV FLHELGLKSF LKTSGGKGLH VIVPLKRLRD
WDTVKDFSQA IVQHLAATIP QRFVAKSGPR NRIGKIFIDY LRNGFGATTV SAWSVRARPG
LGVSVPLAWE ELESLTSASR WSASNIHDRL DKGNDPWSDY EAGRRSILPA MKTLGFKPDK
KTP
//
