ID A0A1G5G045_9FIRM Unreviewed; 457 AA.
AC A0A1G5G045;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE SubName: Full=16S rRNA C967 or C1407 C5-methylase, RsmB/RsmF family {ECO:0000313|EMBL:SCY44906.1};
GN ORFNames=SAMN02910292_01776 {ECO:0000313|EMBL:SCY44906.1};
OS Lachnospiraceae bacterium XBB2008.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX NCBI_TaxID=1520826 {ECO:0000313|EMBL:SCY44906.1, ECO:0000313|Proteomes:UP000199089};
RN [1] {ECO:0000313|EMBL:SCY44906.1, ECO:0000313|Proteomes:UP000199089}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=XBB2008 {ECO:0000313|EMBL:SCY44906.1,
RC ECO:0000313|Proteomes:UP000199089};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01023}.
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DR EMBL; FMVL01000006; SCY44906.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G5G045; -.
DR STRING; 1520826.SAMN02910292_01776; -.
DR Proteomes; UP000199089; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR CDD; cd21147; RsmF_methylt_CTD1; 1.
DR Gene3D; 2.30.130.60; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR031341; Methyltr_RsmF_N.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR027391; Nol1_Nop2_Fmu_2.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR031340; RsmF_methylt_CI.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22807:SF30; 28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED; 1.
DR PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR Pfam; PF17125; Methyltr_RsmF_N; 1.
DR Pfam; PF13636; Methyltranf_PUA; 1.
DR Pfam; PF17126; RsmF_methylt_CI; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000199089};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}.
FT DOMAIN 27..292
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT COILED 294..321
FT /evidence="ECO:0000256|SAM:Coils"
FT ACT_SITE 245
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 123..129
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 147
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 192
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 457 AA; 51764 MW; E06D801BB8701038 CRC64;
MTGDMSFLPE EYLARMSELL KDEYEDFLSS YSNERTYGLR INPLKVDLND EDKIKVILTQ
PGCNIDPEDH VTWCDEGYYY NEDSCPGRSP YHEAGAYYIQ EPSAMAVTSL LDPQPGERIC
DLCAAPGGKT THIAGRMRGR GVLVANEYVP DRAGILSQNV ERMGVSNCLV TCMHPADLSQ
HFMGYFDKVC VDAPCSGEGM FRKDHDACLE WSSDNVDMCA ARQFEILDAA SDMVTSGGVI
VYSTCTFEPD ENERVIETFL KCHPEWTLEE SKRIWPHRMR GEGHFAAKLI KTGMSDKSAR
NEDLKNKNRQ LYNEVARFLH TELGFEISDN RIIYENKDHI YMLPDGISEQ MLRGLRVIRP
GIELVTRHKD RFEPAHSLAM SLRPDEVQNV CDLTYDNAVR YLMGETVNVN DNGCNSISGP
WTLMTINGIS FSWGKKVGET IKNHYPKGLR RDVSIKG
//
