ID A0A1G5HEW3_9CLOT Unreviewed; 447 AA.
AC A0A1G5HEW3;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=Replicative DNA helicase {ECO:0000256|ARBA:ARBA00021957, ECO:0000256|RuleBase:RU362085};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551, ECO:0000256|RuleBase:RU362085};
GN ORFNames=SAMN03080606_01980 {ECO:0000313|EMBL:SCY62039.1};
OS Alkaliphilus peptidifermentans DSM 18978.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Alkaliphilus.
OX NCBI_TaxID=1120976 {ECO:0000313|EMBL:SCY62039.1, ECO:0000313|Proteomes:UP000198636};
RN [1] {ECO:0000313|EMBL:SCY62039.1, ECO:0000313|Proteomes:UP000198636}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 18978 {ECO:0000313|EMBL:SCY62039.1,
RC ECO:0000313|Proteomes:UP000198636};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Participates in initiation and elongation during chromosome
CC replication; it exhibits DNA-dependent ATPase activity.
CC {ECO:0000256|RuleBase:RU362085}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665,
CC ECO:0000256|RuleBase:RU362085};
CC -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC {ECO:0000256|ARBA:ARBA00008428, ECO:0000256|RuleBase:RU362085}.
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DR EMBL; FMUS01000011; SCY62039.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G5HEW3; -.
DR STRING; 1120976.SAMN03080606_01980; -.
DR OrthoDB; 9773982at2; -.
DR Proteomes; UP000198636; Unassembled WGS sequence.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-UniRule.
DR CDD; cd00984; DnaB_C; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR InterPro; IPR007692; DNA_helicase_DnaB.
DR InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR InterPro; IPR016136; DNA_helicase_N/primase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR00665; DnaB; 1.
DR PANTHER; PTHR30153:SF2; REPLICATIVE DNA HELICASE; 1.
DR PANTHER; PTHR30153; REPLICATIVE DNA HELICASE DNAB; 1.
DR Pfam; PF00772; DnaB; 1.
DR Pfam; PF03796; DnaB_C; 1.
DR SUPFAM; SSF48024; N-terminal domain of DnaB helicase; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51199; SF4_HELICASE; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU362085};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU362085};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU362085};
KW Helicase {ECO:0000256|RuleBase:RU362085, ECO:0000313|EMBL:SCY62039.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362085};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU362085}; Primosome {ECO:0000256|RuleBase:RU362085};
KW Reference proteome {ECO:0000313|Proteomes:UP000198636}.
FT DOMAIN 180..445
FT /note="SF4 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51199"
SQ SEQUENCE 447 AA; 50585 MW; F045983BFD140FCC CRC64;
MEGINMFNMI PPHSIEAEQS VLGSMLLDRE AILVALEILK SEDFYKEAHK EIFEAIYNLF
NRSEPVDLIT LTEELKTRGT LEAIGGIPYL TSLASGVPIA TNIKYYADIV QKKATLRNLI
HSSQEIVQLG YSTDVEITEI IEQAQKNIYD LSQNRYNEGL TPISELLSYT FDQIEKLYEN
KKGITGMSTG FLDIDRKLGG FHKSDLILVA ARPAMGKSAF ALNLAQNAAV KAGAAAAIFS
LEMSKEQLML RMLASESMVE LNKIQTGNLN EDEWSKIVQA MIPLSRAQIY FDDSPGITVT
EMRSKCRRLK MEKGLSLVLV DYLQLMQGDK RTENRQQEIS AISRNLKIMA KELDCPVIAL
SQLSRAPELR ADHRPILSDL RESGAIEQDA DLVMFLYRDE YYHPESDKKN LAEVIIAKHR
HGETGTIELI WLGQFQKFLD YEKYRES
//
