ID   A0A1G5J3I6_9ACTN        Unreviewed;       349 AA.
AC   A0A1G5J3I6;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 17.
DE   SubName: Full=Isopenicillin N synthase {ECO:0000313|EMBL:SCY82903.1};
GN   ORFNames=SAMN02745898_10440 {ECO:0000313|EMBL:SCY82903.1};
OS   Streptomyces sp. 136MFCol5.1.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=1172182 {ECO:0000313|EMBL:SCY82903.1, ECO:0000313|Proteomes:UP000199326};
RN   [1] {ECO:0000313|EMBL:SCY82903.1, ECO:0000313|Proteomes:UP000199326}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=136MFCol5.1 {ECO:0000313|EMBL:SCY82903.1,
RC   ECO:0000313|Proteomes:UP000199326};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- PATHWAY: Antibiotic biosynthesis. {ECO:0000256|ARBA:ARBA00004792}.
CC   -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC       family. {ECO:0000256|RuleBase:RU003682}.
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DR   EMBL; FMVI01000004; SCY82903.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1G5J3I6; -.
DR   Proteomes; UP000199326; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR026992; DIOX_N.
DR   InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR   InterPro; IPR027443; IPNS-like_sf.
DR   InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR   PANTHER; PTHR47990; 2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED; 1.
DR   PANTHER; PTHR47990:SF258; FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN; 1.
DR   Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR   Pfam; PF14226; DIOX_N; 1.
DR   PRINTS; PR00682; IPNSYNTHASE.
DR   SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR   PROSITE; PS51471; FE2OG_OXY; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW   Iron {ECO:0000256|RuleBase:RU003682};
KW   Metal-binding {ECO:0000256|RuleBase:RU003682};
KW   Oxidoreductase {ECO:0000256|RuleBase:RU003682};
KW   Reference proteome {ECO:0000313|Proteomes:UP000199326}.
FT   DOMAIN          201..309
FT                   /note="Fe2OG dioxygenase"
FT                   /evidence="ECO:0000259|PROSITE:PS51471"
SQ   SEQUENCE   349 AA;  38294 MW;  8B040F96042D2B85 CRC64;
     MSVLNVESAA PEAAQVTVVD GYVPVIDLSS ARSGGAAERQ LAAVIDEVCR TSGFLVIVGH
     GVPERTISEM YRATREFFAL SAETKAELLA VPADPLMRGF GRQGSLAASN AEAEVEQERA
     LPDISETFTV NRLGEPDADR ALPVDADPAL RTPNRWPELP GFTEAYRSYY AAMEVLAGDI
     MRLFALALDL PEDWFEDKIE QHMTNLTANF YPQQPEPPAP GQLRKGMHSD WGSLTILYQD
     DGPGGLQVLD KAGQWLDVPV IEGSFVVNIG DLMAIWTNNR WVSTVHRVVN PPRELAGKER
     YSVPFFHQPA YDALIECIPT CTGPLNPPRH EPVRSGDYII GKFSRAYGA
//