ID   A0A1G5KST3_9ACTN        Unreviewed;       321 AA.
AC   A0A1G5KST3;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   24-JAN-2024, entry version 16.
DE   SubName: Full=Isopenicillin N synthase {ECO:0000313|EMBL:SCZ03231.1};
GN   ORFNames=SAMN02745898_10754 {ECO:0000313|EMBL:SCZ03231.1};
OS   Streptomyces sp. 136MFCol5.1.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=1172182 {ECO:0000313|EMBL:SCZ03231.1, ECO:0000313|Proteomes:UP000199326};
RN   [1] {ECO:0000313|EMBL:SCZ03231.1, ECO:0000313|Proteomes:UP000199326}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=136MFCol5.1 {ECO:0000313|EMBL:SCZ03231.1,
RC   ECO:0000313|Proteomes:UP000199326};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- PATHWAY: Antibiotic biosynthesis. {ECO:0000256|ARBA:ARBA00004792}.
CC   -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC       family. {ECO:0000256|RuleBase:RU003682}.
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DR   EMBL; FMVI01000007; SCZ03231.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1G5KST3; -.
DR   OrthoDB; 21825at2; -.
DR   Proteomes; UP000199326; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR026992; DIOX_N.
DR   InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR   InterPro; IPR027443; IPNS-like_sf.
DR   InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR   PANTHER; PTHR47990; 2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED; 1.
DR   Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR   Pfam; PF14226; DIOX_N; 1.
DR   PRINTS; PR00682; IPNSYNTHASE.
DR   SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR   PROSITE; PS51471; FE2OG_OXY; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW   Iron {ECO:0000256|RuleBase:RU003682};
KW   Metal-binding {ECO:0000256|RuleBase:RU003682};
KW   Oxidoreductase {ECO:0000256|RuleBase:RU003682};
KW   Reference proteome {ECO:0000313|Proteomes:UP000199326}.
FT   DOMAIN          174..302
FT                   /note="Fe2OG dioxygenase"
FT                   /evidence="ECO:0000259|PROSITE:PS51471"
SQ   SEQUENCE   321 AA;  35087 MW;  38E8B01FDB26FAEA CRC64;
     MSEPRIPTID LRPWLSGDAG ARRETAETVD AALRAAGFLL VTGHGVDLAL RTAIRDTARG
     FFLLPAAAKT PYAVKVGGRG WLGPGAEANG CAEGTRTPPD LKESLSFAAE EPTGDPEIDA
     EWFLPNTWPR EAPALRALVT EYLTQMRTLS DRLLELLGAA LGKPEDFFTR HTTHPTWGFN
     INWYPGTEVV GEPVPGQFRI GPHTDFGTVT VLDRQTGKGG LQVFTDDDGW EDAPYDPEAF
     TINIGDLMAR WTGDRWRSGR HRVLPPPSDA PAEELMSLVY FYECDPRTTV VSVPAPTGRV
     THEPVDAHTY LRAKLDAISV G
//