ID   A0A1G5S6L9_9FIRM        Unreviewed;       460 AA.
AC   A0A1G5S6L9;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 22.
DE   SubName: Full=23S rRNA (Uracil-5-)-methyltransferase RumA {ECO:0000313|EMBL:SCZ81209.1};
GN   ORFNames=SAMN03080599_02660 {ECO:0000313|EMBL:SCZ81209.1};
OS   Acidaminobacter hydrogenoformans DSM 2784.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales;
OC   Eubacteriales Family XII. Incertae Sedis; Acidaminobacter.
OX   NCBI_TaxID=1120920 {ECO:0000313|EMBL:SCZ81209.1, ECO:0000313|Proteomes:UP000199208};
RN   [1] {ECO:0000313|EMBL:SCZ81209.1, ECO:0000313|Proteomes:UP000199208}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 2784 {ECO:0000313|EMBL:SCZ81209.1,
RC   ECO:0000313|Proteomes:UP000199208};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC       ProRule:PRU01024}.
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DR   EMBL; FMWL01000017; SCZ81209.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1G5S6L9; -.
DR   STRING; 1120920.SAMN03080599_02660; -.
DR   OrthoDB; 9804590at2; -.
DR   Proteomes; UP000199208; Unassembled WGS sequence.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   Gene3D; 2.40.50.1070; -; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   NCBIfam; TIGR00479; rumA; 1.
DR   PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR   PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01024}; Reference proteome {ECO:0000313|Proteomes:UP000199208};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01024};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01024}.
FT   DOMAIN          1..58
FT                   /note="TRAM"
FT                   /evidence="ECO:0000259|PROSITE:PS50926"
FT   ACT_SITE        412
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT   ACT_SITE        412
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         289
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         318
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         339
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         385
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   460 AA;  50879 MW;  EE41B853BF5E646A CRC64;
     MKKNQIVNLT IEEMENLQEG IGYIDQKPVY VKGALKGQKV EVRISRNRKL HAEGKLLSVL
     EKSEVEKASF CSHYGECGGC SLQTLPYDTQ VEMKKDWIGG LLEASLSPEV FESIDFTGVL
     PSPKRLAYRN KMEFTFGNAV KGGETNLGLH KKGQFGSVIT TNQCQICDSD FTDILEAALN
     LFKRLGVDHY SKFSHEGVLR HLVVRKGEKT GEILVALSAV GARPEDLKVF VDVLLGLELQ
     DEIVGILYVE NPGKADAVNG EPVVLFGRDH YFDEILGLKF RVSLYSFFQT NTLGAERLYQ
     TAFDGLEDIQ GKYVFDLFSG TGTIAQLLAQ RAERVVGVEI VADAVEAAIA NAAENQLSRC
     EFRVGDVLEI LDHYEGHKPD VIVVDPPRMG IAPKALEKII AFGAPEILYI SCNPKTLVEN
     LSVMSQSGYQ PVKVTGVDMF PHTGHCETIC WLKRRGGNME
//