ID A0A1G5V466_9FIRM Unreviewed; 532 AA.
AC A0A1G5V466;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE RecName: Full=Peptide chain release factor 3 {ECO:0000256|HAMAP-Rule:MF_00072};
DE Short=RF-3 {ECO:0000256|HAMAP-Rule:MF_00072};
GN Name=prfC {ECO:0000256|HAMAP-Rule:MF_00072};
GN ORFNames=SAMN02910343_00383 {ECO:0000313|EMBL:SDA40673.1};
OS Allisonella histaminiformans.
OC Bacteria; Bacillota; Negativicutes; Veillonellales; Veillonellaceae;
OC Allisonella.
OX NCBI_TaxID=209880 {ECO:0000313|EMBL:SDA40673.1, ECO:0000313|Proteomes:UP000199689};
RN [1] {ECO:0000313|EMBL:SDA40673.1, ECO:0000313|Proteomes:UP000199689}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15230 {ECO:0000313|EMBL:SDA40673.1,
RC ECO:0000313|Proteomes:UP000199689};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Increases the formation of ribosomal termination complexes
CC and stimulates activities of RF-1 and RF-2. It binds guanine
CC nucleotides and has strong preference for UGA stop codons. It may
CC interact directly with the ribosome. The stimulation of RF-1 and RF-2
CC is significantly reduced by GTP and GDP, but not by GMP.
CC {ECO:0000256|HAMAP-Rule:MF_00072}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|HAMAP-Rule:MF_00072}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. PrfC subfamily.
CC {ECO:0000256|ARBA:ARBA00009978, ECO:0000256|HAMAP-Rule:MF_00072}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_00072}.
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DR EMBL; FMXA01000004; SDA40673.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G5V466; -.
DR STRING; 209880.SAMN02910343_00383; -.
DR OrthoDB; 9804431at2; -.
DR Proteomes; UP000199689; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR GO; GO:0006449; P:regulation of translational termination; IEA:UniProtKB-UniRule.
DR CDD; cd04169; RF3; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 3.30.70.3280; Peptide chain release factor 3, domain III; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR HAMAP; MF_00072; Rel_fac_3; 1.
DR InterPro; IPR035647; EFG_III/V.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004548; PrfC.
DR InterPro; IPR032090; RF3_C.
DR InterPro; IPR038467; RF3_dom_3_sf.
DR InterPro; IPR041732; RF3_GTP-bd.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR NCBIfam; TIGR00503; prfC; 1.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR PANTHER; PTHR43556; PEPTIDE CHAIN RELEASE FACTOR RF3; 1.
DR PANTHER; PTHR43556:SF2; PEPTIDE CHAIN RELEASE FACTOR RF3; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF16658; RF3_C; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF54980; EF-G C-terminal domain-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00072};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134, ECO:0000256|HAMAP-
KW Rule:MF_00072};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00072}; Protein biosynthesis {ECO:0000256|HAMAP-Rule:MF_00072};
KW Reference proteome {ECO:0000313|Proteomes:UP000199689}.
FT DOMAIN 8..278
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
FT BINDING 17..24
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00072"
FT BINDING 85..89
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00072"
SQ SEQUENCE 532 AA; 59619 MW; 7ED2687FB6CADE61 CRC64;
MTVLDEIKRR RTFAIISHPD AGKTTLTEKL LLYGGAIHLA GSVKARKTSR YAVSDWMEIE
KQRGISVTSS VLNFDYNGCR VNILDTPGHA DFSEDTYRTL MAVDSAVMVI DVAKGVEAQT
KKLFKVCSSR GIPIFTFVNK IDHFGKNPFD LMADIEDVLG IRSCPMNWPI GINGNYTGIF
DRETEIAHLF IKDNAHGTKK LEVVSGKLTD EAITSRLDPD IISSLKDDLE LLDEAGDPFD
AQRVASGELT PMFFGSAMTN FGVEPFLKKF LELAPPPMER KTLEGSVSPE NPAFSGFVFK
IQANMDPKHH DRIAFMRICS GVFEKGMSVL HRQSGKMIRL SQPQQFLATE RTTVEKAYPG
DIIGIFDTGE LGVGDTVCDK KHPVTFSDFP VFPPELFGRL SPVSSMKRKQ FLRGVGQLAQ
EGAIQVYSQP YIMESFIIGA VGQLQFEVLK YRLQKEYNVD VRLEQLPYTT ARWTENNTPS
EDLVGIDNAL VVYDVKKRPV YLLPNEWQAG WLIERNPKVK FLETPPIITV DE
//
