ID A0A1G6B518_9FIRM Unreviewed; 826 AA.
AC A0A1G6B518;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=SAMN02910317_00797 {ECO:0000313|EMBL:SDB15751.1};
OS Ruminococcaceae bacterium FB2012.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
OX NCBI_TaxID=1520817 {ECO:0000313|EMBL:SDB15751.1, ECO:0000313|Proteomes:UP000199364};
RN [1] {ECO:0000313|EMBL:SDB15751.1, ECO:0000313|Proteomes:UP000199364}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FB2012 {ECO:0000313|EMBL:SDB15751.1,
RC ECO:0000313|Proteomes:UP000199364};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; FMXS01000004; SDB15751.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G6B518; -.
DR STRING; 1520817.SAMN02910317_00797; -.
DR Proteomes; UP000199364; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 1.10.4080.10; ADP-ribosylation/Crystallin J1; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR005502; Ribosyl_crysJ1.
DR InterPro; IPR036705; Ribosyl_crysJ1_sf.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF03747; ADP_ribosyl_GH; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF101478; ADP-ribosylglycohydrolase; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000199364};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 689..711
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 341..468
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 480..789
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 826 AA; 92587 MW; 578DA77FB751C73F CRC64;
MIRPITTLSS IINSLESEVF EMIGAIIGDI VGSRFEWNNH RSKDFDLLTH KCFFTDDSVM
SLAVCDALMR SQSDYRDLKI QAVHSMQKVG RPYPHCGYGG SFNRWMYSDN PQPYNSYGNG
AAMRVSGCGY AGQTIDEVKL LSKAVTEVTH NHPEGLKGAE AIAVAVFLAR TGKSLIEIQD
YIIKNYYPLR FTLDSIRDSY RFNETCQDTV PQAFEAFFES TGFEDAIRNA ISIGGDSDTL
AAITGSIAEA YYGVPTNIRK HALTFLDERL LKILLEFENI YPAKIEKTND TGSVGITPST
GKKVKTGGRA EMIESAMNVA DLELDASTAH PEETTSQKLF SHLFEACNIL RGPINQDEYK
SYVTPILFFK RLSDVYDEEI QVALEESGGD EEYASFAENH RFVIPDGCHW QDVREASENV
GVAIVNAMNG IERANPDTLS GVFSSFDDAN WTDKTKLSDE RLKDLIEHMS KLKVGNNNYS
ADVMGDSYEF LIKKFADLSK KNAGEFYTPR SIVKLLIMLL APKAGESVYD PAAGTGGMLI
EAIRFMHGDK LTYGRIYGQE KNLATSAIAR MNLFLHGAKD FKVTQGDTLR SPNYLERGSL
QTFDCVVANP PFSLKNWGAE QFSSDIYGRN IWGCPTDSNG DFAWLQHMVK SMNPKTGRCA
VVLPQGVLFR SGKEGEIRKQ LVESDKLEAI ITMASGVFYS TGVSACILFL NNNKAVLHRG
RICMIDGSEI YTPQRAQNIM TEKDIQTIFD YYTAYEDVLE RVKVVTITDI REKDYSLAIN
NYIEKKEQET VPPEEIRRQY FEAYDEMIEA ETRMRDLLLK GGYVSE
//
