ID A0A1G6DIB3_9FLAO Unreviewed; 754 AA.
AC A0A1G6DIB3;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Aconitate hydratase A {ECO:0000256|ARBA:ARBA00019378};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
DE AltName: Full=Citrate hydro-lyase {ECO:0000256|ARBA:ARBA00029682};
DE AltName: Full=Iron-responsive protein-like {ECO:0000256|ARBA:ARBA00031977};
DE AltName: Full=RNA-binding protein {ECO:0000256|ARBA:ARBA00031081};
GN ORFNames=SAMN03097699_1406 {ECO:0000313|EMBL:SDB44839.1};
OS Flavobacteriaceae bacterium MAR_2010_188.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae.
OX NCBI_TaxID=1250295 {ECO:0000313|EMBL:SDB44839.1, ECO:0000313|Proteomes:UP000199442};
RN [1] {ECO:0000313|EMBL:SDB44839.1, ECO:0000313|Proteomes:UP000199442}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Mar_2010_188 {ECO:0000313|EMBL:SDB44839.1,
RC ECO:0000313|Proteomes:UP000199442};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000256|ARBA:ARBA00004173}.
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LT629302; SDB44839.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G6DIB3; -.
DR STRING; 1250295.SAMN03097699_1406; -.
DR OrthoDB; 9764318at2; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000199442; Chromosome i.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:InterPro.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd01584; AcnA_Mitochondrial; 1.
DR Gene3D; 3.40.1060.10; Aconitase, Domain 2; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR015932; Aconitase_dom2.
DR InterPro; IPR006248; Aconitase_mito-like.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01340; aconitase_mito; 1.
DR PANTHER; PTHR43160; ACONITATE HYDRATASE B; 1.
DR PANTHER; PTHR43160:SF3; ACONITATE HYDRATASE, MITOCHONDRIAL; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000199442}.
FT DOMAIN 34..477
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 557..685
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 754 AA; 82528 MW; C975A81A0FB5B781 CRC64;
MAFDIDMIKQ VYSHMAERVD EARKVVGKPL TLSEKILYSH LWDGKPTQAF RRGKDYVDFA
PDRIACQDAT AQMALLQFMQ AGKPKVAVPT TVHCDHLIQA KQGAEKDLKR ANEVSNEVFD
FLASVSNKYG IGFWKPGAGI IHQVVLENYA FPGGMMIGTD SHTVNAGGLG MVAIGVGGAD
AVDVMAGMAW ELKFPKLIGV KLTGKLSGWT SAKDVILKVA GILTVKGGTG AIVEYFGPGA
KNLSCTGKGT ICNMGAEIGA TTSTFGYDDS MERYLRATDR AEVADEANKI REYLTADDEV
YENPESYFDQ LIEIDLDTLR PHLNGPFTPD LATPVGELGE KARKNGWPIK VDWGLIGSCT
NSSYEDLTRA ASIAKQAVDK NIKAKSDFGI NPGSEQIRYT AERDGLLQIF EDLDATIFTN
ACGPCIGQWD RSDVKGDEKN TIVHSFNRNF SKRADGNPNT HAFVGSPEMV AAIAISGRLD
FDPMNDTLIN EDGVEVKLDE PQGIELPPNG FAVEDAGYID PVEDGSHIKV TVSEDSERLQ
LLEPFEPIGD TITGAKLLIK AFGKCTTDHI SMAGPWLRYR GHLDNISNNM LIGAINAYNK
KTNFVKNQLT GEYDGVPAVQ RAYKAKGIKT IVVGDHNYGE GSSREHAAME PRHLGVAAVL
VKSFARIHET NLKKQGMLGL TFANESDYDL IQEDDTFNFI DLNEFAPDKQ LTVEIVHKDG
LRDLIKVNHT YNDAQIAWYN EGSALNLIKR RNAS
//
