ID   A0A1G6L3Z8_9ACTN        Unreviewed;       731 AA.
AC   A0A1G6L3Z8;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 24.
DE   SubName: Full=3-hydroxyacyl-CoA dehydrogenase / enoyl-CoA hydratase / 3-hydroxybutyryl-CoA epimerase {ECO:0000313|EMBL:SDC38030.1};
GN   ORFNames=SAMN05421872_102111 {ECO:0000313|EMBL:SDC38030.1};
OS   Nocardioides lianchengensis.
OC   Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC   Nocardioidaceae; Nocardioides.
OX   NCBI_TaxID=1045774 {ECO:0000313|EMBL:SDC38030.1, ECO:0000313|Proteomes:UP000199034};
RN   [1] {ECO:0000313|EMBL:SDC38030.1, ECO:0000313|Proteomes:UP000199034}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CGMCC 4.6858 {ECO:0000313|EMBL:SDC38030.1,
RC   ECO:0000313|Proteomes:UP000199034};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC         NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC         Evidence={ECO:0000256|ARBA:ARBA00023693};
CC   -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC       {ECO:0000256|ARBA:ARBA00005005}.
CC   -!- SIMILARITY: In the central section; belongs to the 3-hydroxyacyl-CoA
CC       dehydrogenase family. {ECO:0000256|ARBA:ARBA00007005}.
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DR   EMBL; FMZM01000002; SDC38030.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1G6L3Z8; -.
DR   STRING; 1045774.SAMN05421872_102111; -.
DR   OrthoDB; 5240528at2; -.
DR   UniPathway; UPA00659; -.
DR   Proteomes; UP000199034; Unassembled WGS sequence.
DR   GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR   GO; GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway.
DR   CDD; cd06558; crotonase-like; 1.
DR   Gene3D; 1.10.1040.50; -; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR   InterPro; IPR006108; 3HC_DH_C.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   PANTHER; PTHR43612; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA; 1.
DR   PANTHER; PTHR43612:SF3; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA, MITOCHONDRIAL; 1.
DR   Pfam; PF00725; 3HCDH; 1.
DR   Pfam; PF02737; 3HCDH_N; 1.
DR   Pfam; PF00378; ECH_1; 1.
DR   SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 2.
DR   SUPFAM; SSF52096; ClpP/crotonase; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE   3: Inferred from homology;
KW   Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW   Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW   Lipid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239};
KW   Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW   NAD {ECO:0000256|ARBA:ARBA00023027};
KW   Reference proteome {ECO:0000313|Proteomes:UP000199034}.
FT   DOMAIN          327..505
FT                   /note="3-hydroxyacyl-CoA dehydrogenase NAD binding"
FT                   /evidence="ECO:0000259|Pfam:PF02737"
FT   DOMAIN          508..607
FT                   /note="3-hydroxyacyl-CoA dehydrogenase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00725"
SQ   SEQUENCE   731 AA;  77631 MW;  21F7ABBDF988108C CRC64;
     MSTSTDTQSA VHYDKDADGI VTLTLDDPTS SANTMNQLYI DSMNAAVDRL YAEEADVTGV
     VVASAKKTFF AGGNLKNMVQ AGPDDAADVF ALGETVKAAL RRLEKFPKPV VAAINGAALG
     GGYEICLATQ HRIAWDGPGV IVGLPESSLG LLPGGGGVTR IVRKFGLQAG LMDILLTGAQ
     FKASAAKEKG LVDELVATRE ELVPAAKAWI KANPETTLSP WDAPGYKMPG GTPKSPALAG
     FLPAFPALLR KQTKGAVFPA ARAILSAAVE GAQVDFDTAS RIESRYLTNL VVNQGSKDMI
     QAFFFDLQAI KSGSLRPQGI EPWKATKVGV LGAGMMGAGI AYSCARAGME VVLKDVSDEN
     AAKGKAYSEK LNAKAVSRGK LTEEKSQELL DRITPTGDPA DLAGCDLVIE AVFEDPSLKA
     KVFAEIAPYV NADALLCSNT STLPITELAE GVDRPADFIG LHFFSPVDKM PLVEIIRGKE
     TSDVALAKAY DVVQQIKKTP IVVNDSRGFY TSRVIGFFVN EGLALLAEGQ RPYSIERAAT
     QSGYPVGPLQ LTDELNMELM AKIAKATRDA AERDGTPYSE HPGTAVVDTM IGLDRPSRLK
     GAGFFEYVDG KRGSIWSGLA EQFPIADEQI PFVDMKERML FAEALETAKC FEEGVITSAA
     AANIGSIMGI GFPPMTGGAA QFMTGYEAAD GSIGLAAFVK RADELAETYG ERFRPTSYLR
     DLAERGETFP A
//