ID A0A1G6M130_9BACT Unreviewed; 762 AA.
AC A0A1G6M130;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE SubName: Full=Allosteric NADP-dependent malic enzyme {ECO:0000313|EMBL:SDC48695.1};
GN ORFNames=SAMN05216323_103337 {ECO:0000313|EMBL:SDC48695.1};
OS Williamwhitmania taraxaci.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Williamwhitmaniaceae;
OC Williamwhitmania.
OX NCBI_TaxID=1640674 {ECO:0000313|EMBL:SDC48695.1, ECO:0000313|Proteomes:UP000199452};
RN [1] {ECO:0000313|EMBL:SDC48695.1, ECO:0000313|Proteomes:UP000199452}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A7P-90m {ECO:0000313|EMBL:SDC48695.1,
RC ECO:0000313|Proteomes:UP000199452};
RA Capua I., De Benedictis P., Joannis T., Lombin L.H., Cattoli G.;
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: In the N-terminal section; belongs to the malic enzymes
CC family. {ECO:0000256|ARBA:ARBA00007686}.
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DR EMBL; FMYP01000033; SDC48695.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G6M130; -.
DR STRING; 1640674.SAMN05216323_103337; -.
DR OrthoDB; 9805787at2; -.
DR Proteomes; UP000199452; Unassembled WGS sequence.
DR GO; GO:0016746; F:acyltransferase activity; IEA:InterPro.
DR GO; GO:0004470; F:malic enzyme activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR CDD; cd05311; NAD_bind_2_malic_enz; 1.
DR Gene3D; 3.40.50.10950; -; 1.
DR Gene3D; 3.40.50.10750; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR Gene3D; 3.40.50.10380; Malic enzyme, N-terminal domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR012301; Malic_N_dom.
DR InterPro; IPR037062; Malic_N_dom_sf.
DR InterPro; IPR012302; Malic_NAD-bd.
DR InterPro; IPR045213; Malic_NAD-bd_bact_type.
DR InterPro; IPR012188; ME_PTA.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR042113; P_AcTrfase_dom1.
DR InterPro; IPR042112; P_AcTrfase_dom2.
DR InterPro; IPR002505; PTA_PTB.
DR PANTHER; PTHR43237; NADP-DEPENDENT MALIC ENZYME; 1.
DR PANTHER; PTHR43237:SF4; NADP-DEPENDENT MALIC ENZYME; 1.
DR Pfam; PF00390; malic; 1.
DR Pfam; PF03949; Malic_M; 1.
DR Pfam; PF01515; PTA_PTB; 1.
DR PIRSF; PIRSF036684; ME_PTA; 1.
DR SMART; SM01274; malic; 1.
DR SMART; SM00919; Malic_M; 1.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF53659; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|PIRSR:PIRSR036684-2};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NADP {ECO:0000256|PIRSR:PIRSR036684-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000199452}.
FT DOMAIN 18..151
FT /note="Malic enzyme N-terminal"
FT /evidence="ECO:0000259|SMART:SM01274"
FT DOMAIN 163..399
FT /note="Malic enzyme NAD-binding"
FT /evidence="ECO:0000259|SMART:SM00919"
FT ACT_SITE 94
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-1"
FT BINDING 76..83
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT BINDING 136
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-2"
FT BINDING 137
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-2"
FT BINDING 162
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT BINDING 286
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
SQ SEQUENCE 762 AA; 83041 MW; 032645CF06479249 CRC64;
MEEGLKREAL DYHAQGRPGK IEVVPTKPNS SQKDLSLAYS PGVAAPCLEI EKNPEDAYKY
TAKGNLVAVI SNGTAVLGLG DIGALAGKPV MEGKGLLFKT FADIDVFDIE VDTHDIDKFV
ETVRLIAPTF GGINLEDIKA PECFEIEERL KKLLDIPVMH DDQHGTAIIS SAALLNALTI
NGKKIEDLIV VVNGAGAAAV ACAKLYFALG IRAENLVMCD SKGAISTRRK DINSIKAQFA
TQRTVDTLEE AMVGADMFLG LSAANVLTTE MVQSMAKEPI VFALANPDPE IAYDLAMKAR
PDIIFATGRS DYPNQVNNVL GFPYIFRGAL DVRAKTINEE MKVAAVRALA SLAKEVVPDI
VANAYNKKTL AFGRDYLIPT PLDPRLISTI SVAVAQAAVK SGVARKTIED WDAYKTELEQ
RLGYDNAIIR TVRDRTRNSG KRIVFAEGGR LKVLQAAQQI LQEGIATPIL LGSAEKIRKI
SLDNNIDLNG ATIIEFYGDA EACRREVFAK LFFEKRKRKG ITYQEAIDSM YDRNQFGIMM
VEVGDADAFI SGYSSKYSTT IKPALQIVGT DNPGNHIAGM YMVMTKQGPI FLADTTVNDQ
PNAETLVATT LLVDRAVRRF NIEPVIAMVS YSNFGSVPTG QPEISRRAVE ILHANHPEII
VDGEVQANFA LNKKIRMEKF PFSKWGEKRV NTLVFPDLAS GNISYKLLQE LGGFEVVGPI
LVGIAKPVHV VPIESSVREI VNMATIAVFD SICTLEGNCG CE
//
