ID   A0A1G6MSJ6_9BACT        Unreviewed;       365 AA.
AC   A0A1G6MSJ6;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   24-JAN-2024, entry version 18.
DE   SubName: Full=KamA family protein {ECO:0000313|EMBL:SDC58533.1};
DE   SubName: Full=KamA family radical SAM protein {ECO:0000313|EMBL:TGG87359.1};
GN   ORFNames=E4650_08630 {ECO:0000313|EMBL:TGG87359.1}, SAMN04488588_1368
GN   {ECO:0000313|EMBL:SDC58533.1};
OS   Geotoga petraea.
OC   Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae; Geotoga.
OX   NCBI_TaxID=28234 {ECO:0000313|EMBL:SDC58533.1, ECO:0000313|Proteomes:UP000199322};
RN   [1] {ECO:0000313|EMBL:SDC58533.1, ECO:0000313|Proteomes:UP000199322}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WG14 {ECO:0000313|EMBL:SDC58533.1,
RC   ECO:0000313|Proteomes:UP000199322};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:TGG87359.1, ECO:0000313|Proteomes:UP000297288}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HO-Geo1 {ECO:0000313|EMBL:TGG87359.1,
RC   ECO:0000313|Proteomes:UP000297288};
RA   Grouzdev D.S., Semenova E.M., Sokolova D.S., Tourova T.P., Poltaraus A.B.,
RA   Nazina T.N.;
RT   "Draft genome sequence data and analysis of a Fermenting Bacterium, Geotoga
RT   petraea strain HO-Geo1, isolated from heavy-oil petroleum reservoir in
RT   Russia.";
RL   Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000256|ARBA:ARBA00001933};
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DR   EMBL; FMYV01000005; SDC58533.1; -; Genomic_DNA.
DR   EMBL; SRME01000005; TGG87359.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1G6MSJ6; -.
DR   STRING; 28234.SAMN04488588_1368; -.
DR   OrthoDB; 9768064at2; -.
DR   Proteomes; UP000199322; Unassembled WGS sequence.
DR   Proteomes; UP000297288; Unassembled WGS sequence.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd01335; Radical_SAM; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR003739; Lys_aminomutase/Glu_NH3_mut.
DR   InterPro; IPR007197; rSAM.
DR   NCBIfam; TIGR00238; KamA family radical SAM protein; 1.
DR   PANTHER; PTHR30538:SF0; L-LYSINE 2,3-AMINOMUTASE AQ_1632-RELATED; 1.
DR   PANTHER; PTHR30538; LYSINE 2,3-AMINOMUTASE-RELATED; 1.
DR   Pfam; PF13353; Fer4_12; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   PIRSF; PIRSF004911; DUF160; 1.
DR   SFLD; SFLDG01070; PLP-dependent; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   4: Predicted;
KW   4Fe-4S {ECO:0000256|PIRSR:PIRSR004911-1};
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|PIRSR:PIRSR004911-
KW   1};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR004911-1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000199322};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT   DOMAIN          84..300
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000259|PROSITE:PS51918"
FT   BINDING         98
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004911-1"
FT   BINDING         102
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004911-1"
FT   BINDING         105
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004911-1"
SQ   SEQUENCE   365 AA;  42882 MW;  62897C7BAE068E3E CRC64;
     MYPEYLINID KVSKLSEEEK VALKEVTQKY KFRSNEYYLS LVDWEDPEDP IRKIIIPQMG
     ELEEWGSLDS SSEKNYTVQR GFQHKYRDTA LLLVNDVCGG FCRFCFRKRL FINPGEEVSR
     NVEKEIEYIK DHKEITNVLL TGGDPLLLST KKLKDIITKI REIDHVKIIR IGSKLFSFNP
     YRIIEDESLL DLIEENSTRE KRIYFMMHFN HPKEIQKPSI EAIDKVLKSG AIAVNQTPLV
     KGINDNSKVL SELFKKLSFY GVPPYYVFQG RPVKGNKPFI VPVEKGFTIF LKSIASISGL
     AKRPRFVMSH KTGKIEVSSM TKENIIFRYH RAVDEDNYKK FFVYKRNPNA YWYDDYKDLK
     EIYQF
//