ID A0A1G6PNJ0_9BURK Unreviewed; 400 AA.
AC A0A1G6PNJ0;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=3-oxoadipyl-CoA thiolase {ECO:0000256|ARBA:ARBA00012233};
DE EC=2.3.1.174 {ECO:0000256|ARBA:ARBA00012233};
GN ORFNames=SAMN05192589_103294 {ECO:0000313|EMBL:SDC81753.1};
OS Paracidovorax valerianellae.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Paracidovorax.
OX NCBI_TaxID=187868 {ECO:0000313|EMBL:SDC81753.1, ECO:0000313|Proteomes:UP000198781};
RN [1] {ECO:0000313|EMBL:SDC81753.1, ECO:0000313|Proteomes:UP000198781}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 16619 {ECO:0000313|EMBL:SDC81753.1,
RC ECO:0000313|Proteomes:UP000198781};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + succinyl-CoA = 3-oxoadipyl-CoA + CoA;
CC Xref=Rhea:RHEA:19481, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC ChEBI:CHEBI:57292, ChEBI:CHEBI:57348; EC=2.3.1.174;
CC Evidence={ECO:0000256|ARBA:ARBA00000708};
CC -!- PATHWAY: Aromatic compound metabolism. {ECO:0000256|ARBA:ARBA00005211}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC {ECO:0000256|ARBA:ARBA00010982, ECO:0000256|RuleBase:RU003557}.
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DR EMBL; FMZC01000003; SDC81753.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G6PNJ0; -.
DR STRING; 187868.SAMN05192589_103294; -.
DR OrthoDB; 8558405at2; -.
DR Proteomes; UP000198781; Unassembled WGS sequence.
DR GO; GO:0033812; F:3-oxoadipyl-CoA thiolase activity; IEA:UniProtKB-EC.
DR GO; GO:0019619; P:3,4-dihydroxybenzoate catabolic process; IEA:InterPro.
DR CDD; cd00751; thiolase; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR012793; PcaF.
DR InterPro; IPR002155; Thiolase.
DR InterPro; IPR016039; Thiolase-like.
DR InterPro; IPR020615; Thiolase_acyl_enz_int_AS.
DR InterPro; IPR020610; Thiolase_AS.
DR InterPro; IPR020617; Thiolase_C.
DR InterPro; IPR020613; Thiolase_CS.
DR InterPro; IPR020616; Thiolase_N.
DR NCBIfam; TIGR01930; AcCoA-C-Actrans; 1.
DR NCBIfam; TIGR02430; pcaF; 1.
DR PANTHER; PTHR43853; 3-KETOACYL-COA THIOLASE, PEROXISOMAL; 1.
DR PANTHER; PTHR43853:SF2; 3-OXOADIPYL-COA_3-OXO-5,6-DEHYDROSUBERYL-COA THIOLASE; 1.
DR Pfam; PF02803; Thiolase_C; 1.
DR Pfam; PF00108; Thiolase_N; 1.
DR PIRSF; PIRSF000429; Ac-CoA_Ac_transf; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS00098; THIOLASE_1; 1.
DR PROSITE; PS00737; THIOLASE_2; 1.
DR PROSITE; PS00099; THIOLASE_3; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|RuleBase:RU003557};
KW Reference proteome {ECO:0000313|Proteomes:UP000198781};
KW Transferase {ECO:0000256|RuleBase:RU003557, ECO:0000313|EMBL:SDC81753.1}.
FT DOMAIN 5..267
FT /note="Thiolase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00108"
FT DOMAIN 275..399
FT /note="Thiolase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02803"
FT ACT_SITE 90
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
FT ACT_SITE 356
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
FT ACT_SITE 386
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
SQ SEQUENCE 400 AA; 41560 MW; D88A6230E57B12ED CRC64;
MTQAFICDAI RTPFGRYGGA LSSVRTDDLG AIPLKALMAR NPGVDWAAVT DVLYGCANQA
GEDNRNVARM AALLAGLPID VPGATINRLC GSGLDAVGTA ARAIKAGEAG LMIAGGVESM
SRAPFVMPKA ETAFSRTNAV YDTTIGWRFV NKLMKAQYGV DSMPETAENV ADDYRIEREA
QDRMALASQL KAVAAQQAGH LAREITPVHV PQKKGDPVVV DKDEHPRETS LEALARLKPV
VREGGTVTAG NASGVNDGAC ALLLADEASA AKHGLTPKAR VVGMATAGVA PRVMGIGPAP
ATQKVLQLTG LTLEQMDVIE LNEAFAAQGI AVLRQLGLQD DDERVNAWGG AIALGHPLGA
SGARLATTAV NRLHATGGRY ALATMCIGVG QGIAVVLERV
//
