ID A0A1G6VGX0_9GAMM Unreviewed; 532 AA.
AC A0A1G6VGX0;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 15.
DE RecName: Full=malate synthase {ECO:0000256|ARBA:ARBA00012636};
DE EC=2.3.3.9 {ECO:0000256|ARBA:ARBA00012636};
GN ORFNames=SAMN04488509_10383 {ECO:0000313|EMBL:SDD52940.1};
OS Aquimonas voraii.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Xanthomonadales;
OC Rhodanobacteraceae; Aquimonas.
OX NCBI_TaxID=265719 {ECO:0000313|EMBL:SDD52940.1, ECO:0000313|Proteomes:UP000199603};
RN [1] {ECO:0000313|EMBL:SDD52940.1, ECO:0000313|Proteomes:UP000199603}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 16957 {ECO:0000313|EMBL:SDD52940.1,
RC ECO:0000313|Proteomes:UP000199603};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00001699};
CC -!- SIMILARITY: Belongs to the malate synthase family.
CC {ECO:0000256|ARBA:ARBA00006394}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FNAG01000003; SDD52940.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G6VGX0; -.
DR STRING; 265719.SAMN04488509_10383; -.
DR Proteomes; UP000199603; Unassembled WGS sequence.
DR GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR CDD; cd00727; malate_synt_A; 1.
DR Gene3D; 3.20.20.360; Malate synthase, domain 3; 1.
DR Gene3D; 1.20.1220.12; Malate synthase, domain III; 1.
DR InterPro; IPR044856; Malate_synth_C_sf.
DR InterPro; IPR011076; Malate_synth_sf.
DR InterPro; IPR006252; Malate_synthA.
DR InterPro; IPR001465; Malate_synthase_TIM.
DR InterPro; IPR048355; MS_C.
DR InterPro; IPR048356; MS_N.
DR InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR NCBIfam; TIGR01344; malate_syn_A; 1.
DR PANTHER; PTHR42902; MALATE SYNTHASE; 1.
DR PANTHER; PTHR42902:SF1; MALATE SYNTHASE 1-RELATED; 1.
DR Pfam; PF20659; MS_C; 1.
DR Pfam; PF20656; MS_N; 1.
DR Pfam; PF01274; MS_TIM-barrel; 1.
DR PIRSF; PIRSF001363; Malate_synth; 1.
DR SUPFAM; SSF51645; Malate synthase G; 1.
PE 3: Inferred from homology;
KW Glyoxylate bypass {ECO:0000256|ARBA:ARBA00022435};
KW Reference proteome {ECO:0000313|Proteomes:UP000199603};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532}.
FT DOMAIN 15..70
FT /note="Malate synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF20656"
FT DOMAIN 162..405
FT /note="Malate synthase TIM barrel"
FT /evidence="ECO:0000259|Pfam:PF01274"
FT DOMAIN 412..528
FT /note="Malate synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF20659"
FT ACT_SITE 166
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
FT ACT_SITE 446
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
SQ SEQUENCE 532 AA; 59804 MW; 2DDEFA32CA2391D2 CRC64;
MAPSSAAQSF STQAQADPRV QRVLTAEAVA LLTDLHRRFE PTRQRLLQAR RDWQARIDAG
ALPDFRPETR EIRESDWTVA PSPEALQDRR VEITGPVDRK MIINALNSGA KVFMADFEDS
SSPTRRNMLD GQINLMDAVA GTIEFTSPEG KRYALKPEGP ATLIVRPRGW HLDEANFRVD
GQPMSGSLFD MALFAFHNGR SLHARDRGPY FYLPKMQAFE EAALWDEAMA FVENRLGLPV
GCMKATVLIE TLPAVFEMHE ILHALRCRIV GLNCGRWDYI FSYIKTFRRH ADRVLPDRSQ
VTMTVPFLKA YSELLIQTCH RRGAHAMGGM AAQIPIPSDP VANEEALARV RADKLREVTA
GHDGTWVAHP GLIPVAREIF DKHMPTPNQL HVRRDDVQVN RDLLIHPSIG TITREGFYAN
IDVCVRYLAA WLDGMGCVPI HNLMEDAATA EISRAQLWQW LHTPDQRFDD GDPIDMALFE
RAIGTVEQRL PRSGLPGQGR LKEAAQLLAE LTRADSLEDF ITLPAYGRLD HA
//
