ID A0A1G7D815_9DEIN Unreviewed; 1671 AA.
AC A0A1G7D815;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=SAMN04488243_10243 {ECO:0000313|EMBL:SDE47712.1};
OS Thermus arciformis.
OC Bacteria; Deinococcota; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=482827 {ECO:0000313|EMBL:SDE47712.1, ECO:0000313|Proteomes:UP000199446};
RN [1] {ECO:0000313|EMBL:SDE47712.1, ECO:0000313|Proteomes:UP000199446}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 1.6992 {ECO:0000313|EMBL:SDE47712.1,
RC ECO:0000313|Proteomes:UP000199446};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; FNBC01000002; SDE47712.1; -; Genomic_DNA.
DR STRING; 482827.SAMN04488243_10243; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000199446; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd00081; Hint; 2.
DR CDD; cd12113; PHP_PolIIIA_DnaE3; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 2.
DR Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR01443; intein_Cterm; 1.
DR NCBIfam; TIGR01445; intein_Nterm; 1.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF14890; Intein_splicing; 1.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF55608; Homing endonucleases; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 4: Predicted;
KW Autocatalytic cleavage {ECO:0000256|ARBA:ARBA00022813};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Protein splicing {ECO:0000256|ARBA:ARBA00023000};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 931..1070
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000259|PROSITE:PS50819"
FT DOMAIN 1243..1265
FT /note="Intein C-terminal splicing"
FT /evidence="ECO:0000259|PROSITE:PS50818"
SQ SEQUENCE 1671 AA; 186388 MW; C0C4AA465B750825 CRC64;
MGRKLRFAHL HQHTQFSLLD GAAKLSDLLK WVKETTPEDP ALAITDHGNL FGAVEFYKKA
TAMGIKPILG YEAYVAAESR FDRKRGKGLD GGYFHLTLLA KDFRGYQNLV RLASRAYLEG
FYEKPRIDRE ILREHAEGLI ALSGCLGAEI PQFILQDRLD LAEARLNEYL SIFGDRFFIE
IQNHGLPEQR KVNEVLKEFA RKYGLGMVAT NDGHYVRKED ARAHEVLLAI QSKSTLDDPE
RWRFPCDEFY VKTPEEMRAM LPEEEWGDEP FDNTVEIARM CDVDLPIGDK MVYRIPRFPL
PEGRTEAQYL RELTFKGLLR RYPDRITEGF YREVFRLLGK LPPHGDGEAL AEALAGVEEE
AWERLMKALP PLEGVREWTA EAILHRALYE LSVMERMGFP GYFLIVQDYI NWARSHGISV
GPGRGSAAGS LVAYAVGITN IDPLRFGLLF ERFLNPERVS MPDIDTDFSD RERDRVIQYV
RERYGEDKVA QIGTLGSLAS KAALKDVARV YGIPHKRAEE LAKLIPVQFG KPKPLAEAIQ
VVPELKAEME KDPKVREVLE VAMRLEGLNR HASVHAAGVV IAAEPLTDLV PLMRDQEGRP
VTQYDMGAVE ALGLLKMDFL GLRTLTFLDE ARRIVRESRG VELDYDRLPL DDAKTFELLS
RGETKGVFQL ESGGMTATVR GLKPRRIEDI IALVSLYRPG PMEHIPTYIR RHHGQEPVSY
SEFPHAEKYL KPILDETYGI PVYQEQIMQI ASQVAGYSLG EADLLRRAMG KKRVEEMQKH
RERFVRGAKE RGVPEEEANR LFDMLEAFAN YGFNKCLPAR ARVVDWRTGR VVRVGEIVRG
EVEEVWVVSL DEARLRLVPR PVVAAFPSGK AQVYALRTAT GRVLEATANH PVYTPRGWRP
LGALAPGDYV ALPRHLPYRP SAHLEAHELD LLGFALAEGN LRHPSGFYLY TSSEEELAAM
EEALRAFPNT RTRVVWRRGV AHVYVGRMDR RQEAGAVAFL RRMGLLGLDA KTKRLPEAVF
GLPPEEVARF LGRLWTGDGG VDPKGRLIHY ATASKELASG VQHLLLRLGL QSRLVEKRFS
GGYKGYAVYL LGGLEAARRF AETVGPYLVG KRRQDLEALL ASWGEAGQST KDVLPLAFLE
EVREAVAQGQ AAALLREAGL RPGRGRRGLS RATVGRLAAL TGSLSARTLI SPHGTPGVGL
CEGSFGAPAL AQARAGYLAL LRLAEAEVYW DRVEAVEPLG EEEVFDLTVE GTHTFVAEDV
IVHNSHAAAY SLLSYQTAYV KAHYPVEFMA ALLSVERHDS DKVAEYIRDA RAMGIEVLPP
DINRSGFDFK VVGEEILFGL SAVKNVGEAA AEAILKERER GGPFKSLGDF LKRLDEKVVN
KRTLESLIKA GAMDAFGDRA RLLASLDPLL RWAAGVRERE RSGMWGLFAE VEEPPLQEAP
PLDEITRLRY EKEALGIYVS GHPVLRYPGL REAASCTLEE LPEFVRGLPP RSRVLLAGMV
EDVVRKPTRS GGMMARFTLS DETGALEVVV FGRAYEGVSP KLKEDTPLLV LAEVEREEGG
LRVIAQAAWT HEEVAEAAKA LEVEVDHALL DEKGVALLKS LLDEHPGPLP LYVRVQGPFG
EALFSLREAR VGEEALGALE AEGFRAYLVP DREVFLQANG GNGSKEEAVP F
//
