ID A0A1G7EVX2_9NOCA Unreviewed; 559 AA.
AC A0A1G7EVX2;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE SubName: Full=3-oxosteroid 1-dehydrogenase {ECO:0000313|EMBL:SDE67810.1};
GN ORFNames=SAMN05444580_12811 {ECO:0000313|EMBL:SDE67810.1};
OS Rhodococcus tukisamuensis.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Rhodococcus.
OX NCBI_TaxID=168276 {ECO:0000313|EMBL:SDE67810.1, ECO:0000313|Proteomes:UP000199417};
RN [1] {ECO:0000313|EMBL:SDE67810.1, ECO:0000313|Proteomes:UP000199417}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 11308 {ECO:0000313|EMBL:SDE67810.1,
RC ECO:0000313|Proteomes:UP000199417};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
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DR EMBL; FNAB01000028; SDE67810.1; -; Genomic_DNA.
DR RefSeq; WP_072846480.1; NZ_FNAB01000028.1.
DR AlphaFoldDB; A0A1G7EVX2; -.
DR STRING; 168276.SAMN05444580_12811; -.
DR Proteomes; UP000199417; Unassembled WGS sequence.
DR GO; GO:0033765; F:steroid dehydrogenase activity, acting on the CH-CH group of donors; IEA:UniProt.
DR GO; GO:0008202; P:steroid metabolic process; IEA:UniProt.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR InterPro; IPR003953; FAD-binding_2.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR027477; Succ_DH/fumarate_Rdtase_cat_sf.
DR PANTHER; PTHR43400:SF7; FAD_BINDING_2 DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR43400; FUMARATE REDUCTASE; 1.
DR Pfam; PF00890; FAD_binding_2; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR SUPFAM; SSF56425; Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain; 1.
PE 4: Predicted;
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000199417}.
FT DOMAIN 13..535
FT /note="FAD-dependent oxidoreductase 2 FAD binding"
FT /evidence="ECO:0000259|Pfam:PF00890"
SQ SEQUENCE 559 AA; 60108 MW; 079F1CAC3A3C5B16 CRC64;
MTASEQNWDH EVDFLVVGSG CGGLTGALAG ADRGLDTLII EKATVYGGTT ALSGGNIWIP
NNPTLTREGL ADSREDVRRY LDAVVCDTVP SANLDAFIDN GPQTMEFLEN TSRHLRFQWC
TGYSDYHPEQ PGGRAAGRTI EPLPFDLKEL GEDEKLLRPG ALATPAGLYI TSKEFAKLNM
VMRTWAGRRA ALGTGWRAVK SLILRRHMET LGQALIGRLR LAVKEAGVPL WLGTPMQSLV
TDDSGAVVGV VAERGGAEVR IRTRKGVLLA SGGFEQGEQM RKQYLREGGK ENYSAGSVDN
TGDGIVAGEQ VGAALDLMDD AWWMPSFQRP DGIMHVLVSE RSIPRSIIVD QSGRRFTNEA
APYVTFVHEQ IAGRHEPIWF IFDGKAKNRY QFGGVMPGQK FPKSWFATGL MHQADTLAEL
ASAIGLPSAQ LAETVQRFNG FALGGKDEDF GRGESAYDNY YGDPTLPAPA MDVLDQAPYY
AVRMQAGDLG TKGGLVYNEH AQVLRADGSV IGGLYATGNT SAAVMGNDYA GAGATIGPAM
VFGYVGARHC AASEKSADD
//
