ID   A0A1G7L2I6_9SPHN        Unreviewed;       942 AA.
AC   A0A1G7L2I6;
DT   18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT   18-JAN-2017, sequence version 1.
DT   27-MAR-2024, entry version 25.
DE   SubName: Full=Insulinase family protein {ECO:0000313|EMBL:MWC43471.1};
DE   SubName: Full=Zinc protease {ECO:0000313|EMBL:SDF43717.1};
GN   ORFNames=GQR91_07345 {ECO:0000313|EMBL:MWC43471.1},
GN   SAMN05216557_103355 {ECO:0000313|EMBL:SDF43717.1};
OS   Sphingomonas carotinifaciens.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingomonas.
OX   NCBI_TaxID=1166323 {ECO:0000313|EMBL:SDF43717.1, ECO:0000313|Proteomes:UP000323502};
RN   [1] {ECO:0000313|EMBL:SDF43717.1, ECO:0000313|Proteomes:UP000323502}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=S7-754 {ECO:0000313|EMBL:SDF43717.1,
RC   ECO:0000313|Proteomes:UP000323502};
RA   Varghese N., Submissions S.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:MWC43471.1, ECO:0000313|Proteomes:UP000436801}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 27347 {ECO:0000313|EMBL:MWC43471.1,
RC   ECO:0000313|Proteomes:UP000436801};
RA   Zheng J.;
RL   Submitted (DEC-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|ARBA:ARBA00001947};
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
CC       {ECO:0000256|ARBA:ARBA00007261}.
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DR   EMBL; WSUT01000005; MWC43471.1; -; Genomic_DNA.
DR   EMBL; FNBI01000003; SDF43717.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1G7L2I6; -.
DR   OrthoDB; 9811314at2; -.
DR   Proteomes; UP000323502; Unassembled WGS sequence.
DR   Proteomes; UP000436801; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0008233; F:peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 4.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   PANTHER; PTHR11851; METALLOPROTEASE; 1.
DR   PANTHER; PTHR11851:SF229; PEPTIDASE M16 DOMAIN PROTEIN; 1.
DR   Pfam; PF00675; Peptidase_M16; 2.
DR   Pfam; PF05193; Peptidase_M16_C; 2.
DR   SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 4.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000313|EMBL:SDF43717.1};
KW   Protease {ECO:0000313|EMBL:SDF43717.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000323502};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           22..942
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5036019157"
FT   DOMAIN          50..196
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          204..382
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
FT   DOMAIN          514..660
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          668..842
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
SQ   SEQUENCE   942 AA;  99925 MW;  AF3B507B198D1E10 CRC64;
     MRCLRAALLL SVATLPATLS AQEQPQPPAA SARIPSIPFT ERTLANGLRV YAIRDTTTPN
     VSVQVWYDVG GRDDPRGRSG FAHLFEHLMF KATRNLVQEQ MDRLTEDVGG YNNASTGDDY
     TNYFEVIPAN HLQRLLFAEA DRMASLVVEP TSFASERDVV KEEYRQGFAR PYGKLFQTYL
     PEISYSVHPY ARGVIGDVAN LDSATIDDVR AFHATYYRPD NAILVVAGNF DPAQLNGWVD
     QYFAPIKRPA GEIPRVTVTE PPRERPVSRT VYEANTPLPA VLLSWQLPPD RDADVPALQV
     LDAIMATGEN SRLYRSLVYR DELAQSAQTS FDTRAGTGTY AVFAIMAGGK DAAVGEAALR
     REVARFRDEA VTPAELAEAK NEILTAAIKR RETAEGKAFL LAQNVIINDD PTASDKQLAA
     VQRVTAADIQ RVARKYLGDN QSAELRYLPE QANKTGDTVT VAPTVVTRPL TAPADIAIVT
     PAPAGQRILP PPPAAPVKPV LPQVAETRLA NGLRVITVER HDLPLVTASL VAVGGAATDP
     AGKAGASSLS AGLMTMGTTT RSAAEIARAV ESLGGSIGSD ASRDGASVDL TVTSDQLAPA
     MTILADVATH PAFAAEEIER ARTQTIDGVA VAMKNPAQLS GLVASRVVFG NQPYGTPLEG
     TPTSLKTLTQ ADLKAAYQRT WQPGQAALVL VGDITPAAAR TLAERYLGTW QGKGTAAAVP
     GDRAAFPAPR VVVVDMPDAG QAGVVVARPG IARGDARYYP LAVANTVLGG GFSSRLNQEV
     RIKRGLAYGA SSGLQAGRRP GLVAARTQTK NPSAAEVVTL ISAEMEKLGK TPVPQAELAT
     RQAVLVGNFG RTVETTDGIA GLLGEYVVQS VPLDELQRYT AKVEGVDPAA VQAAAAALLD
     PKAASIVVVG DARQFLPALK ARYPQVEVVP AAAVNLDSAS LK
//