ID A0A1G7MCG3_9BACL Unreviewed; 811 AA.
AC A0A1G7MCG3;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE SubName: Full=Nitrite reductase (NADH) large subunit {ECO:0000313|EMBL:SDF59294.1};
GN ORFNames=SAMN04488542_11358 {ECO:0000313|EMBL:SDF59294.1};
OS Fontibacillus panacisegetis.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Fontibacillus.
OX NCBI_TaxID=670482 {ECO:0000313|EMBL:SDF59294.1, ECO:0000313|Proteomes:UP000198972};
RN [1] {ECO:0000313|EMBL:SDF59294.1, ECO:0000313|Proteomes:UP000198972}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 28129 {ECO:0000313|EMBL:SDF59294.1,
RC ECO:0000313|Proteomes:UP000198972};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|PIRNR:PIRNR037149};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- COFACTOR:
CC Name=siroheme; Xref=ChEBI:CHEBI:60052;
CC Evidence={ECO:0000256|ARBA:ARBA00001929};
CC -!- PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
CC {ECO:0000256|ARBA:ARBA00005096}.
CC -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S domain
CC family. {ECO:0000256|ARBA:ARBA00010429}.
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DR EMBL; FNBG01000013; SDF59294.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G7MCG3; -.
DR STRING; 670482.SAMN04488542_11358; -.
DR UniPathway; UPA00653; -.
DR Proteomes; UP000198972; Unassembled WGS sequence.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0050661; F:NADP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008942; F:nitrite reductase [NAD(P)H] activity; IEA:UniProtKB-UniRule.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-UniRule.
DR CDD; cd19943; NirB_Fer2_BFD-like_1; 1.
DR CDD; cd19944; NirB_Fer2_BFD-like_2; 1.
DR Gene3D; 3.30.390.30; -; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR Gene3D; 3.30.413.10; Sulfite Reductase Hemoprotein, domain 1; 1.
DR Gene3D; 3.90.480.10; Sulfite Reductase Hemoprotein;Domain 2; 1.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR016156; FAD/NAD-linked_Rdtase_dimer_sf.
DR InterPro; IPR005117; NiRdtase/SiRdtase_haem-b_fer.
DR InterPro; IPR036136; Nit/Sulf_reduc_fer-like_dom_sf.
DR InterPro; IPR012744; Nitri_red_NirB.
DR InterPro; IPR017121; Nitrite_Rdtase_lsu.
DR InterPro; IPR006067; NO2/SO3_Rdtase_4Fe4S_dom.
DR InterPro; IPR045854; NO2/SO3_Rdtase_4Fe4S_sf.
DR InterPro; IPR006066; NO2/SO3_Rdtase_FeS/sirohaem_BS.
DR InterPro; IPR041575; Rubredoxin_C.
DR NCBIfam; TIGR02374; nitri_red_nirB; 1.
DR PANTHER; PTHR43809; NITRITE REDUCTASE (NADH) LARGE SUBUNIT; 1.
DR PANTHER; PTHR43809:SF1; NITRITE REDUCTASE (NADH) LARGE SUBUNIT; 1.
DR Pfam; PF04324; Fer2_BFD; 2.
DR Pfam; PF01077; NIR_SIR; 1.
DR Pfam; PF03460; NIR_SIR_ferr; 1.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR Pfam; PF18267; Rubredoxin_C; 1.
DR PIRSF; PIRSF037149; NirB; 1.
DR PRINTS; PR00368; FADPNR.
DR PRINTS; PR00411; PNDRDTASEI.
DR PRINTS; PR00397; SIROHAEM.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 2.
DR SUPFAM; SSF56014; Nitrite and sulphite reductase 4Fe-4S domain-like; 1.
DR SUPFAM; SSF55124; Nitrite/Sulfite reductase N-terminal domain-like; 1.
DR PROSITE; PS00365; NIR_SIR; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|PIRNR:PIRNR037149};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW ECO:0000256|PIRNR:PIRNR037149}; Heme {ECO:0000256|ARBA:ARBA00022617};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nitrate assimilation {ECO:0000256|ARBA:ARBA00023063,
KW ECO:0000256|PIRNR:PIRNR037149};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000198972}.
FT DOMAIN 8..284
FT /note="FAD/NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF07992"
FT DOMAIN 320..387
FT /note="NADH-rubredoxin oxidoreductase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF18267"
FT DOMAIN 424..472
FT /note="BFD-like [2Fe-2S]-binding"
FT /evidence="ECO:0000259|Pfam:PF04324"
FT DOMAIN 487..536
FT /note="BFD-like [2Fe-2S]-binding"
FT /evidence="ECO:0000259|Pfam:PF04324"
FT DOMAIN 562..624
FT /note="Nitrite/Sulfite reductase ferredoxin-like"
FT /evidence="ECO:0000259|Pfam:PF03460"
FT DOMAIN 632..768
FT /note="Nitrite/sulphite reductase 4Fe-4S"
FT /evidence="ECO:0000259|Pfam:PF01077"
SQ SEQUENCE 811 AA; 88309 MW; 452B49F859047EA3 CRC64;
MNMREKKKLV MVGNGMAGVR TIEHVLKLAP DTYEITIFGA EPYPNYNRIM LSSVLAGGAD
MQEIVINDWD WYTENHIHLH TGDAVTVIDT TNRYVMSESG VRAPYDALIL ATGSNPFMLP
LPGANKEGVI GFRDIHDCNL MMEASKKYSK AAVIGGGLLG LEAARGLLHL GMDVSVIHIN
SYLMERQLDE PAAKLLQKEL EAQGMKFLMQ KQTSGIVGGR RAEGLQFADG TELSADLIVM
AVGIKPNIEL AKSSGIEVGR GIIVNDYLET SIPEIYAVGE CAEHRGIAYG LVAPLYEQGN
VLAKRLAGLD APGYEGSVTS TKLKVSGVDV FSAGYFNESP QTRSLRIQDD IEGTYKKIVL
QDDRLVGAVL FGDISDGAEL FSKIKSGECV AGKEKELLLG YPAGQPAGGN SQSARLEAMA
NDEIICGCNG VTKGTIVDAV RNGCSSVGEI KSCTKASGSC GGCKPLVAGL LELYGDGPSG
EPVKEGICGC TTLSRDEIVE SIKTMGLKSV KEVIHVLDWN QPEGCSKCRP SLNYYLGMIW
PTEYRDEKES RFANERYHAN IQKDGTFSVV PRIYGGVTSP ADLKRIAEVA EKYEVPMVKF
TGGQRIDLLG VKKDDLPKVW EELDMPSGHA YGKTLRTVKT CVGSTFCRFG TQDSISMGIR
LEKAFERLNT PGKVKLAVSG CPRNCAEATI KDLGVVAIDG GWEIYVGGNG GVKVRAADLL
CTVKSEDEVM EWTAAYLQYY REQASWNERT AQWLERVGLD TIKAALSDAE ERQALAERIK
ETLSHTTDPW KEIIENKELR KNFESLQGVQ V
//
