ID   A0A1G7T5G8_9ACTN        Unreviewed;       423 AA.
AC   A0A1G7T5G8;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 20.
DE   SubName: Full=tRNA/tmRNA/rRNA uracil-C5-methylase, TrmA/RlmC/RlmD family {ECO:0000313|EMBL:SDG30546.1};
GN   ORFNames=SAMN05660324_2317 {ECO:0000313|EMBL:SDG30546.1};
OS   Klenkia brasiliensis.
OC   Bacteria; Actinomycetota; Actinomycetes; Geodermatophilales;
OC   Geodermatophilaceae; Klenkia.
OX   NCBI_TaxID=333142 {ECO:0000313|EMBL:SDG30546.1, ECO:0000313|Proteomes:UP000198863};
RN   [1] {ECO:0000313|EMBL:SDG30546.1, ECO:0000313|Proteomes:UP000198863}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 44526 {ECO:0000313|EMBL:SDG30546.1,
RC   ECO:0000313|Proteomes:UP000198863};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC       ProRule:PRU01024}.
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DR   EMBL; FNCF01000003; SDG30546.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1G7T5G8; -.
DR   Proteomes; UP000198863; Unassembled WGS sequence.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR   GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR   Gene3D; 2.40.50.1070; -; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR030391; MeTrfase_TrmA_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR   PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01024};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01024};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01024}.
FT   DOMAIN          6..65
FT                   /note="TRAM"
FT                   /evidence="ECO:0000259|PROSITE:PS50926"
FT   ACT_SITE        370
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT   ACT_SITE        370
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         242
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         271
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         295
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         343
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   423 AA;  44044 MW;  327D4D914107BE53 CRC64;
     MSTDRDSWVG QRFEVTVGPV AHGGHCVARH EGRVVFVRHA LPGERVVVAV TEDRHPGYCR
     ADAVEVLDAS PDRVERPCKA SGPGRCGGCD WQHVDPAAQR RLKADVVTEQ FGRLAGMDVA
     LDVEELPGGP LRWRSRARFA VDRAGRPGLR PHRSRDVVVL EDCPITVEPA VAAVLGRRWD
     GAGAVDVAVD SVGGVTTTVL DRRGHARSTK VARPTGAEVT GARAERRAWH RDWQVEGTGF
     WQVHPAAADA LVGAVAGFVG VRPGERVLDL YAGAGLFGGS LAAGVGAGGQ VVCVESDAGA
     CAAAADNLAD LPQAEVWQGD VDAEGLAGLL AELDGPPDVV VLDPPRSGAG REVSEVLAGS
     GARAVAYVAC DPASLARDVA VFAGAGWRLA GLRAFDAFPM TAHVECVALL LPGAQPVASA
     ERS
//