ID   A0A1G7WV15_9FLAO        Unreviewed;       471 AA.
AC   A0A1G7WV15;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 20.
DE   SubName: Full=23S rRNA m(5)U-1939 methyltransferase {ECO:0000313|EMBL:SDG75787.1};
GN   ORFNames=SAMN04488027_106149 {ECO:0000313|EMBL:SDG75787.1};
OS   Psychroflexus sediminis.
OC   Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC   Flavobacteriaceae; Psychroflexus.
OX   NCBI_TaxID=470826 {ECO:0000313|EMBL:SDG75787.1, ECO:0000313|Proteomes:UP000199296};
RN   [1] {ECO:0000313|EMBL:SDG75787.1, ECO:0000313|Proteomes:UP000199296}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 19803 {ECO:0000313|EMBL:SDG75787.1,
RC   ECO:0000313|Proteomes:UP000199296};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC       ProRule:PRU01024}.
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DR   EMBL; FNCW01000006; SDG75787.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1G7WV15; -.
DR   STRING; 470826.SAMN04488027_106149; -.
DR   OrthoDB; 9804590at2; -.
DR   Proteomes; UP000199296; Unassembled WGS sequence.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR   GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   Gene3D; 2.40.50.1070; -; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR030391; MeTrfase_TrmA_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   NCBIfam; TIGR00479; rumA; 1.
DR   PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR   PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01024}; Reference proteome {ECO:0000313|Proteomes:UP000199296};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01024};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01024}.
FT   DOMAIN          4..64
FT                   /note="TRAM"
FT                   /evidence="ECO:0000259|PROSITE:PS50926"
FT   ACT_SITE        428
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT   ACT_SITE        428
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         302
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         331
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         352
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         401
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   471 AA;  54242 MW;  F5DCE56F3572D6FA CRC64;
     MARRKNTKKT FENVEVLDAG SKGKGIGKAS DGRVLIIDQV VPGDVVDVTT YKKRKSYYQA
     KPLRFVELSP KRTEPVCQHF GVCGGCKWQN MAYQHQLFYK EKEVLENLKR IGKIDIVETK
     PILGSEEIYF YRNKMEFSFS NSRWLTESEI SSGIDIQDKN ALGFHIPGMW DKILNIEKCH
     LQEDPSNDIR NKIRDFATEN ELDFFNPREQ TGLLRTLMIR TSSTGEFMIL IQFYQENKAK
     RELLLDYIIQ EFPDVTSLQY VINEKGNDTI YDQEVINYFG RDFIYEEMEG LKFKINAKSF
     YQTNSEQAYE LYKITREFAD LSGDELVYDL YTGTGTIAQF VAKKAKHVVG IEAIPEAIED
     AKANAKLNGI ENTSFFAGDM KKIFTAGFIA EHGQPDVIIT DPPREGMHKE VVEQILNVRP
     KRIVYVSCNS ATQARDLQLM DAHYQVEKSQ AVDMFPQTHH VENVVCLRLR E
//