ID A0A1G7XDL7_9SPHI Unreviewed; 935 AA.
AC A0A1G7XDL7;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=SAMN05192573_10523 {ECO:0000313|EMBL:SDG82203.1};
OS Mucilaginibacter gossypii.
OC Bacteria; Bacteroidota; Sphingobacteriia; Sphingobacteriales;
OC Sphingobacteriaceae; Mucilaginibacter.
OX NCBI_TaxID=551996 {ECO:0000313|EMBL:SDG82203.1, ECO:0000313|Proteomes:UP000199705};
RN [1] {ECO:0000313|EMBL:SDG82203.1, ECO:0000313|Proteomes:UP000199705}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Gh-67 {ECO:0000313|EMBL:SDG82203.1,
RC ECO:0000313|Proteomes:UP000199705};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
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DR EMBL; FNCG01000005; SDG82203.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G7XDL7; -.
DR STRING; 551996.SAMN05192573_10523; -.
DR Proteomes; UP000199705; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 2..263
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 342..523
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 692..899
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 935 AA; 105141 MW; 7EBC6F9989815CC5 CRC64;
MKKLFLLDGM ALIYRAHFAL SKTPRFTSNG LNTSAMMGFT NTLLDVLKKE APTHMAVVFD
TEAPTERHTD FEGYKAHREA MPEDLAKALP YVIKIILGFN IPVITSDGYE ADDIIGTLAK
KAEQKGYTVY CMTPDKDFGQ LVSDNIFIYK PARMGNEMEI QGVKEILAKW EIERVDQVID
ILGLWGDAVD GIPGIPGIGE KTAKALIKQY GSMENIFEHS HELKGKQREN VEQFKEQGLM
SKKLATILLN VPVELDEEGL EVCAPSKELL EPLFAELEFR TLGKRVFGDD FSITELKPAG
TQIDLFGNPT ATGRTTMTVD VEDIREDLII AAKNINNTPH EYHLADTLEK RAELINILAQ
QPTICFDTET TGTDPNHCEL VGLSFSVKPR QGWYVPVPAD QAGAQSIVTE FKAVLENENI
GKIGQNIKFD ILMLKWYNIE VKGTLFDTML AHYILDPDTR HNMDILSENY LGYKPVSITE
LIGPKGKNQG NMRDVDFEKI KEYAAEDADI TLQLKDIFEP KLKEVEGEKL VNEIEHPLIY
VLADMEYEGV KIDHDTLREF SKQLETDIAQ YEKTVYEKAG VKFNIASPKQ LGEVLFEKLM
LDPKAKKTKT GQYQTGEDVL LSLAAKSDIV RDILDFRQLQ KLKSTYVDAL PQMVNPKTGR
VHTSYNQAVA ATGRLSSVNP NLQNIPIRTE RGREVRKAFI PRDSNHTIVS ADYSQIELRI
IAEISKDPNM MQAFINNHDI HTATAANVYG IGLDEVTSDQ RRNAKAVNFG IIYGQSAFGL
SQSLGIPRKE AAEIIEQYFV QFAGIKQYMS DTMNFARENG YVCTLMGRRR YLRDINSANA
TVRGFAERNA INAPIQGSAA DMIKIAMINI HKELKAQKLD TRMTMQVHDE LVFDVPNHEV
EIVKPIIMHH MKNAIKTEVP IMVEIGTGLN WLEAH
//
