ID A0A1G8E4X5_9BACI Unreviewed; 717 AA.
AC A0A1G8E4X5;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 15.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN ORFNames=SAMN05192534_108137 {ECO:0000313|EMBL:SDH64770.1};
OS Alteribacillus persepolensis.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Alteribacillus.
OX NCBI_TaxID=568899 {ECO:0000313|EMBL:SDH64770.1, ECO:0000313|Proteomes:UP000199163};
RN [1] {ECO:0000313|EMBL:SDH64770.1, ECO:0000313|Proteomes:UP000199163}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 21632 {ECO:0000313|EMBL:SDH64770.1,
RC ECO:0000313|Proteomes:UP000199163};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
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DR EMBL; FNDK01000008; SDH64770.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G8E4X5; -.
DR SMR; A0A1G8E4X5; -.
DR STRING; 568899.SAMN05192534_108137; -.
DR Proteomes; UP000199163; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR049117; pulA_all-beta.
DR InterPro; IPR011840; PulA_typeI.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF21653; pulA_all-beta; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000199163}.
FT DOMAIN 222..618
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 717 AA; 82760 MW; 2D88594AC769F572 CRC64;
MTSNHHAINS DLIAWLEDLY TVKVKGDTAF ASNQWRIVKK ENGKETLIDA ACFIEEGFVY
LQRKEPYQAG VRYEVVSPDN RRAHVLSGKA VRTLLFDDLY AYDGDDLGAR CHRGETLFAV
WAPTAEEVHL VIHRHTKHEK EAVHVMKRTI RGVWRMELQG EHHGLCYTYR VFVNGVWQET
PDPYAASSTL NGDQSVVIDW RKIPDKAYAI PKTAITDAII YEVHVRDFTA HLYSGVKQKG
KYLGWTERDT KTPNGCTSAL SYIKELGVTH VQLLPVHDFG SVDETKEPPA YNWGYDPVQH
FVPEGSYVVN KHQPEARIRE LKQLIDTLHQ HGIGVILDVV LNHFYKIETA SLEKIVPGYY
FRYDDYGLLS NGTGVGNDTA SERYMMRKYI IDCLLHWIRH YQVDGFRFDL MGIHDVTTMQ
EVSCRLREEK PSVFLYGEGW NMNTVLADKQ KASINQAPQL GKRVGFFNDR FRNTVKGNIF
GSLGFIHGER ADVDVDNVAF VMRGSIRWEE KKKTGLFTAP SQSINYVECH DNHTLWDQLN
IQGDMSLEEK RKMHRLTLVM VLTAQGVPFL HGGQEFFRTK YGVENSYNAP VWINQFDWEQ
REIHNENVEF LKNLIQIRKQ FDGFRMQTTA VIERRFQQLA QPKDLFIFKI HKVEHKENAK
DLYVLFNHTK NPRKVVLPEK GIYQVIVDGE RASLIPLKTI HSNTIEVQPV SAVILYK
//
