ID A0A1G8S0W1_9RHOB Unreviewed; 414 AA.
AC A0A1G8S0W1;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=Sarcosine oxidase subunit beta {ECO:0000256|ARBA:ARBA00044150};
DE EC=1.5.3.24 {ECO:0000256|ARBA:ARBA00044044};
DE AltName: Full=Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta {ECO:0000256|ARBA:ARBA00044216};
DE AltName: Full=Tetrameric sarcosine oxidase subunit beta {ECO:0000256|ARBA:ARBA00044295};
GN ORFNames=SAMN04487993_102276 {ECO:0000313|EMBL:SDJ22782.1};
OS Salipiger marinus.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Roseobacteraceae; Salipiger.
OX NCBI_TaxID=555512 {ECO:0000313|EMBL:SDJ22782.1, ECO:0000313|Proteomes:UP000199093};
RN [1] {ECO:0000313|EMBL:SDJ22782.1, ECO:0000313|Proteomes:UP000199093}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 26424 {ECO:0000313|EMBL:SDJ22782.1,
RC ECO:0000313|Proteomes:UP000199093};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + O2 + sarcosine = (6R)-5,10-
CC methylene-5,6,7,8-tetrahydrofolate + glycine + H2O2;
CC Xref=Rhea:RHEA:70455, ChEBI:CHEBI:15379, ChEBI:CHEBI:15636,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:57305, ChEBI:CHEBI:57433,
CC ChEBI:CHEBI:57453; EC=1.5.3.24;
CC Evidence={ECO:0000256|ARBA:ARBA00043745};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O2 + sarcosine = formaldehyde + glycine + H2O2;
CC Xref=Rhea:RHEA:13313, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:16842, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:57433; Evidence={ECO:0000256|ARBA:ARBA00043809};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the SoxB family.
CC {ECO:0000256|ARBA:ARBA00043973}.
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DR EMBL; FNEJ01000022; SDJ22782.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G8S0W1; -.
DR STRING; 555512.SAMN04487993_102276; -.
DR OrthoDB; 9815989at2; -.
DR Proteomes; UP000199093; Unassembled WGS sequence.
DR GO; GO:0008115; F:sarcosine oxidase activity; IEA:InterPro.
DR GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR Gene3D; 3.30.9.10; D-Amino Acid Oxidase, subunit A, domain 2; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR006076; FAD-dep_OxRdtase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR001763; Rhodanese-like_dom.
DR InterPro; IPR006278; SoxB.
DR NCBIfam; TIGR01373; soxB; 1.
DR PANTHER; PTHR13847:SF287; FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1; 1.
DR PANTHER; PTHR13847; SARCOSINE DEHYDROGENASE-RELATED; 1.
DR Pfam; PF01266; DAO; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR PROSITE; PS50206; RHODANESE_3; 1.
PE 3: Inferred from homology;
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022643};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Reference proteome {ECO:0000313|Proteomes:UP000199093}.
FT DOMAIN 35..79
FT /note="Rhodanese"
FT /evidence="ECO:0000259|PROSITE:PS50206"
SQ SEQUENCE 414 AA; 45131 MW; 6FFB4365613A0750 CRC64;
MTRYSAFAIA REAARYHSGW TRAWRKPEPK PRYDVVIVGG GGHGLATAYY LGKNFGITNV
AIVEKGWLGG GNTGRNTTII RSNYLQDPSA AIYEKARSLY ETLSQDLNYN VMFSPRGLMM
LAQTEHELRG YQRTVHANRL QGVETEMIGP REIKRLCPIM NVDGPRYPVL GALWQARGGT
ARHDAVAWGY ARACSDMGMD ILQNTEVTAI RSEGGRVRGI ATTRGEIGCD RLGVVVAGHS
GVLAEMAGFR LPIESVALQA LVSEPIKPCM DVVVMANTVH GYMSQSDKGE MVIGGGTDGF
VNYTQRGSFH HIEETVRALI ETFPMISRLK MLRQWGGIVD VTGDRSPILS KTPLEGCFFN
CGWGTGGFKA IPGSGWGFAE LMAKGASPLC AEFGLDRFRE GRFIDESVAA GVAH
//
