ID   A0A1G8T7F8_9ACTN        Unreviewed;       590 AA.
AC   A0A1G8T7F8;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 19.
DE   SubName: Full=3-oxosteroid 1-dehydrogenase {ECO:0000313|EMBL:SDJ36875.1};
GN   ORFNames=SAMN05421806_10173 {ECO:0000313|EMBL:SDJ36875.1};
OS   Streptomyces indicus.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=417292 {ECO:0000313|EMBL:SDJ36875.1, ECO:0000313|Proteomes:UP000199155};
RN   [1] {ECO:0000313|EMBL:SDJ36875.1, ECO:0000313|Proteomes:UP000199155}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CGMCC 4.5727 {ECO:0000313|EMBL:SDJ36875.1,
RC   ECO:0000313|Proteomes:UP000199155};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; FNFF01000001; SDJ36875.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1G8T7F8; -.
DR   STRING; 417292.SAMN05421806_10173; -.
DR   OrthoDB; 9813348at2; -.
DR   Proteomes; UP000199155; Unassembled WGS sequence.
DR   GO; GO:0033765; F:steroid dehydrogenase activity, acting on the CH-CH group of donors; IEA:UniProt.
DR   GO; GO:0008202; P:steroid metabolic process; IEA:UniProt.
DR   Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR   InterPro; IPR003953; FAD-binding_2.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR027477; Succ_DH/fumarate_Rdtase_cat_sf.
DR   InterPro; IPR006311; TAT_signal.
DR   InterPro; IPR019546; TAT_signal_bac_arc.
DR   NCBIfam; TIGR01409; TAT_signal_seq; 1.
DR   PANTHER; PTHR43400:SF7; FAD_BINDING_2 DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR43400; FUMARATE REDUCTASE; 1.
DR   Pfam; PF00890; FAD_binding_2; 1.
DR   SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR   SUPFAM; SSF56425; Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain; 1.
DR   PROSITE; PS51318; TAT; 1.
PE   4: Predicted;
KW   Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Reference proteome {ECO:0000313|Proteomes:UP000199155};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..39
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           40..590
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5011449818"
FT   DOMAIN          49..574
FT                   /note="FAD-dependent oxidoreductase 2 FAD binding"
FT                   /evidence="ECO:0000259|Pfam:PF00890"
SQ   SEQUENCE   590 AA;  61873 MW;  C3CE585E6AAEF08F CRC64;
     MPENTSAEPP VRPSRRTFLG ASAAASVAVA TGLPATAQAA APPVAGEFDV VVVGAGAAGM
     TAAMTAAARG LSCVVVEKAG TYGGSAARSG AGIWIPNNSV IRAAGVPDTP QKAATYLAAV
     VGDEVPRSRQ QAFLDHGPAM LDFVMARSPL KFRYMEGYSD YYPELPGGLP GGRSIEPDLL
     DGRVLGAELD RLNGAYMPVP DGMVVFSADY KWLTLSLVNA KGFAVANECL ARATNAALHG
     VKPLTMGQAL AGGLRAGLLR ANVPVWLNCP LTDLHLENGA VRGVIVTRNG APALVRARRG
     VIVGSGGFEH NAAMRAQYQQ QPIGTDWTVG AKENTGDGIR AGARAGAALG LMDDAWWGPA
     IPLPNEPYFC LAERTLPGGL LINQAGARFV NEAAPYSDVV HVMYEKNPTA PDIPAWLIVD
     QNYRNRYLFK DIAPTFVFPD SWYESGAVHK AWTLDQLARD IGVPGAALKS TVHRFNSLAR
     DGKDPDFHRG DSAYDHYYTD PGVTPNSCLA PLWLAPFYAF KIVPGDLGTK GGLLTDARAR
     VLRPDGSVIP GLYAAGNASS AVMGRSYAGA GSTIGPAMTF GYIAANDLAA
//