ID A0A1G9E9R4_9PROT Unreviewed; 517 AA.
AC A0A1G9E9R4;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=SAMN05192566_2245 {ECO:0000313|EMBL:SDK72786.1};
OS Methylophilus rhizosphaerae.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
OC Methylophilaceae; Methylophilus.
OX NCBI_TaxID=492660 {ECO:0000313|EMBL:SDK72786.1, ECO:0000313|Proteomes:UP000198629};
RN [1] {ECO:0000313|EMBL:SDK72786.1, ECO:0000313|Proteomes:UP000198629}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBMB127 {ECO:0000313|EMBL:SDK72786.1,
RC ECO:0000313|Proteomes:UP000198629};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; FNFX01000004; SDK72786.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G9E9R4; -.
DR STRING; 492660.SAMN05192566_2245; -.
DR OrthoDB; 9784823at2; -.
DR Proteomes; UP000198629; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 10..157
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 171..483
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 517 AA; 57547 MW; B948B62CDDCB2C7F CRC64;
MTSAQQRAEL QRQIWQIANE VRGSVDGWDF KQYVLGTLFY RFISENFASY IEGGDESVNY
AELADDVITP EIKDDAIKTK GYFIYPSQLF VNVATKASKN HNLNTDLAKI FSAIESSANG
YPSELDIKGL FADFDTTSNR LGNSVPEKNT RLAAVLKGVA GLNLGSFEDS KIDLFGDAYE
FLISNYAANA GKSGGEFFTP QNVSKLIAQL AMHKQDTVNK IYDPAAGSGS LLLQAKRQFD
DHIIEEGFFG QEINHTTYNL ARMNMFLHNI NYDKFHIALG DTLRNPQFGD QKPFDAIVSN
PPYSVNWIGS DDPTLINDER FAPAGVLAPK SKADFAFVLH ALSYLSSKGR AAIVCFPGIF
YRGGAEQKIR KYLVDNNYVE TIISLAPNLF YGTSIAVTIL VLSKHKTDTK TQFIDASALY
KKETNNNVLT VEGENNHIEQ IMKVFDSKSD IDHFAQSVDY EKIVANEYNL SVSSYVEAKD
TREVIDINKL NAEIRTTVAR IDQLRANIDA IVAEIHG
//
