ID A0A1G9FVH3_9RHOB Unreviewed; 760 AA.
AC A0A1G9FVH3;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE SubName: Full=Malate dehydrogenase (Oxaloacetate-decarboxylating)(NADP+) {ECO:0000313|EMBL:SDK92436.1};
GN ORFNames=SAMN04487971_104113 {ECO:0000313|EMBL:SDK92436.1};
OS Paracoccus chinensis.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Paracoccaceae; Paracoccus.
OX NCBI_TaxID=525640 {ECO:0000313|EMBL:SDK92436.1, ECO:0000313|Proteomes:UP000199555};
RN [1] {ECO:0000313|EMBL:SDK92436.1, ECO:0000313|Proteomes:UP000199555}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 1.7655 {ECO:0000313|EMBL:SDK92436.1,
RC ECO:0000313|Proteomes:UP000199555};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SIMILARITY: In the N-terminal section; belongs to the malic enzymes
CC family. {ECO:0000256|ARBA:ARBA00007686}.
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DR EMBL; FNGE01000004; SDK92436.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G9FVH3; -.
DR STRING; 525640.SAMN04487971_104113; -.
DR OrthoDB; 9805787at2; -.
DR Proteomes; UP000199555; Unassembled WGS sequence.
DR GO; GO:0016746; F:acyltransferase activity; IEA:InterPro.
DR GO; GO:0004470; F:malic enzyme activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR CDD; cd05311; NAD_bind_2_malic_enz; 1.
DR Gene3D; 3.40.50.10950; -; 1.
DR Gene3D; 3.40.50.10750; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR Gene3D; 3.40.50.10380; Malic enzyme, N-terminal domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR015884; Malic_enzyme_CS.
DR InterPro; IPR012301; Malic_N_dom.
DR InterPro; IPR037062; Malic_N_dom_sf.
DR InterPro; IPR012302; Malic_NAD-bd.
DR InterPro; IPR045213; Malic_NAD-bd_bact_type.
DR InterPro; IPR012188; ME_PTA.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR042113; P_AcTrfase_dom1.
DR InterPro; IPR042112; P_AcTrfase_dom2.
DR InterPro; IPR002505; PTA_PTB.
DR PANTHER; PTHR43237; NADP-DEPENDENT MALIC ENZYME; 1.
DR PANTHER; PTHR43237:SF4; NADP-DEPENDENT MALIC ENZYME; 1.
DR Pfam; PF00390; malic; 1.
DR Pfam; PF03949; Malic_M; 1.
DR Pfam; PF01515; PTA_PTB; 1.
DR PIRSF; PIRSF036684; ME_PTA; 1.
DR SMART; SM01274; malic; 1.
DR SMART; SM00919; Malic_M; 1.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF53659; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS00331; MALIC_ENZYMES; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR036684-2};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NADP {ECO:0000256|PIRSR:PIRSR036684-3};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 26..159
FT /note="Malic enzyme N-terminal"
FT /evidence="ECO:0000259|SMART:SM01274"
FT DOMAIN 171..407
FT /note="Malic enzyme NAD-binding"
FT /evidence="ECO:0000259|SMART:SM00919"
FT ACT_SITE 102
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-1"
FT BINDING 84..91
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT BINDING 144
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-2"
FT BINDING 145
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-2"
FT BINDING 170
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT BINDING 295
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
SQ SEQUENCE 760 AA; 81554 MW; 71C6718CA3816200 CRC64;
MTDSNQPQRS RITPEEALAY HLEPCPGKLE IVPSTPMATQ RDLSLAYSPG VAVPVEAIAA
RPETAYDYTT KGNLVAVVSN GTAILGLGNL GALASKPVME GKAVLFKRFA DVNAIDIELD
TEDPEEIIRA VRLMGPSFGG INLEDIKAPE CFIIESRLKE MMDIPVFHDD QHGTAVICAA
GLLNALELSG KRIEDVKIVL NGAGAAGIAC LELLKAMGAR HENCIMADTK GVIYQGRTEG
MNQWKSAHAV ATEARTLADA MVGCDVFLGV SAKGAVTQDM VKSMAPNPVI FAMANPDPEI
TPEEAHAVRP DAIVATGRSD YPNQVNNVLG FPYLFRGALD IHARAINDEM KIACARALAE
LAREDVPDEV AVAYGRKLSF GRDYIIPTPF DPRLIHVVPP AVAKAGMDTG AARRPIIDME
GYVQTLRQRM DPTASILQGI HARARQAQAR MIFAEGDDPR VLRAAVAWQR GGLGRALVVG
REEDVAEKLA AEGMADALRE LEIVSALTSP HLNAYHETLY KRLQRQGVDR EDALKLARRD
RHVFAALMLA HGHGDALVTG ATRKASHVLS QVGHVFDLRP QDGAVGITGV LHKGRIVLVA
DTLVHEWPEA EDLALIATRG AHVARALGLE PRVAFLSFST FGHPISERAI KMAEAPKVLD
RQGADFEYEG EMTVDVALNP EAAARYPFSR LTGPANVLVM PARHSASISV RLLQEMAGAT
VIGPILTGIP RPIQICGIGS TVNDILNMAA MAAGRVGQVA
//
