ID A0A1G9MAE7_9PSED Unreviewed; 952 AA.
AC A0A1G9MAE7;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=phosphoenolpyruvate--protein phosphotransferase {ECO:0000256|ARBA:ARBA00012232};
DE EC=2.7.3.9 {ECO:0000256|ARBA:ARBA00012232};
GN ORFNames=SAMN05216186_12838 {ECO:0000313|EMBL:SDL71236.1};
OS Pseudomonas indica.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=137658 {ECO:0000313|EMBL:SDL71236.1, ECO:0000313|Proteomes:UP000198706};
RN [1] {ECO:0000313|EMBL:SDL71236.1, ECO:0000313|Proteomes:UP000198706}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 21544 {ECO:0000313|EMBL:SDL71236.1,
RC ECO:0000313|Proteomes:UP000198706};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-
CC L-histidyl-[protein] + pyruvate; Xref=Rhea:RHEA:23880, Rhea:RHEA-
CC COMP:9745, Rhea:RHEA-COMP:9746, ChEBI:CHEBI:15361, ChEBI:CHEBI:29979,
CC ChEBI:CHEBI:58702, ChEBI:CHEBI:64837; EC=2.7.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00000683};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the PEP-utilizing enzyme family.
CC {ECO:0000256|ARBA:ARBA00007837}.
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DR EMBL; FNFD01000028; SDL71236.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G9MAE7; -.
DR STRING; 137658.SAMN05216186_12838; -.
DR Proteomes; UP000198706; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:InterPro.
DR CDD; cd00367; PTS-HPr_like; 1.
DR CDD; cd00211; PTS_IIA_fru; 1.
DR Gene3D; 3.30.1340.10; HPr-like; 1.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR Gene3D; 3.50.30.10; Phosphohistidine domain; 1.
DR Gene3D; 1.10.274.10; PtsI, HPr-binding domain; 1.
DR InterPro; IPR000032; HPr-like.
DR InterPro; IPR035895; HPr-like_sf.
DR InterPro; IPR008279; PEP-util_enz_mobile_dom.
DR InterPro; IPR018274; PEP_util_AS.
DR InterPro; IPR000121; PEP_util_C.
DR InterPro; IPR023151; PEP_util_CS.
DR InterPro; IPR036637; Phosphohistidine_dom_sf.
DR InterPro; IPR016152; PTrfase/Anion_transptr.
DR InterPro; IPR006318; PTS_EI-like.
DR InterPro; IPR002178; PTS_EIIA_type-2_dom.
DR InterPro; IPR008731; PTS_EIN.
DR InterPro; IPR001020; PTS_HPr_His_P_site.
DR InterPro; IPR036618; PtsI_HPr-bd_sf.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR NCBIfam; TIGR01417; PTS_I_fam; 1.
DR PANTHER; PTHR46244; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR PANTHER; PTHR46244:SF3; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR Pfam; PF05524; PEP-utilisers_N; 1.
DR Pfam; PF00391; PEP-utilizers; 1.
DR Pfam; PF02896; PEP-utilizers_C; 1.
DR Pfam; PF00381; PTS-HPr; 1.
DR Pfam; PF00359; PTS_EIIA_2; 1.
DR PRINTS; PR00107; PHOSPHOCPHPR.
DR PRINTS; PR01736; PHPHTRNFRASE.
DR SUPFAM; SSF47831; Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain; 1.
DR SUPFAM; SSF55594; HPr-like; 1.
DR SUPFAM; SSF55804; Phoshotransferase/anion transport protein; 2.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR SUPFAM; SSF52009; Phosphohistidine domain; 1.
DR PROSITE; PS00742; PEP_ENZYMES_2; 1.
DR PROSITE; PS00370; PEP_ENZYMES_PHOS_SITE; 1.
DR PROSITE; PS51094; PTS_EIIA_TYPE_2; 1.
DR PROSITE; PS00372; PTS_EIIA_TYPE_2_HIS; 1.
DR PROSITE; PS51350; PTS_HPR_DOM; 1.
DR PROSITE; PS00369; PTS_HPR_HIS; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Reference proteome {ECO:0000313|Proteomes:UP000198706};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 2..142
FT /note="PTS EIIA type-2"
FT /evidence="ECO:0000259|PROSITE:PS51094"
FT DOMAIN 282..372
FT /note="HPr"
FT /evidence="ECO:0000259|PROSITE:PS51350"
FT REGION 630..652
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 952 AA; 101667 MW; EC7041D00AB9A57D CRC64;
MLELTAEQIR MGQSAADKSQ ALRLMASTLE TDGLVAPGYL AGLEAREAQG STYLGQGIAI
PHGTPETRDQ VFTTGVRLLQ FPEGVDWGDS QQVYLAIGIA ARSDEHLHLL QLLTRALGEA
DLGEALRQAD SAERIIQLLQ GAPQELALDA QLVGLGLAVD DFDELLWQGA HLLKRADCAD
SGFAAKLQQT EPLPLGDGLW WLHSEQAVKR PGLAFVTPEK AIVHQGQPLT GLFCLASLGE
AHRALLERLC ALLIEGRGQV FSQATSQRAV LEALGGELPV DWPSVRVPLA NPHGLHARPA
NVLTHLAKSF DGEIRVRIAD SGATAVSAKS LSKLLSLGAR RGQVLEFLAE PSIAADALPA
LQAAVEEGLG EEIEPLPAAG EGETFESPAE APVAPAAGSI LPAIAASPGI AIGPAYVRVA
QRFEYPQRGE SPAAERDRLG SALTEVRDEI TGLIQRSESK TIREIFVTHL EMLDDPALSE
DVAARLQQGE SAEAAWAAVI EAAASQQEGL HDALLAERAA DLRDVGRRVL AQLCDVEAAV
EPDEPYILVM DEVGPSDVAR LDRARVAGIL TARGGATAHS AIVARTLGIP ALVGAGPAVL
ALAPRTLLLL DAEHGRLHVA PDANALRRAE QARSEREQRL RAAQAQRLEP ARTRDGHELE
VCANLGDTKG VTAAVEQGAD GVGLLRSEFV FMAHAQAPDQ ATQEAEYRHV LDVLAGRPLV
VRTLDVGGDK PLPYWPIPEE ANPYLGLRGI RLCLQRPQVL ETQLRALLSA AGERPLRIMF
PMVGSIEEWR QARDMALRLR EEIPVTDLQL GIMVEVPSAA LLAPVLAREV DFFSVGTNDL
TQYTLAIDRG HPTLSAQADG LHPAVLQLID MTVRAAHAHG KWVGVCGELA ADPHAVPLLV
GLGVDELSVS ARSIAEVKAR VRELTLAEAR TLAQRALELP SATEVRALLE VR
//
