ID A0A1G9R4S7_9FIRM Unreviewed; 293 AA.
AC A0A1G9R4S7;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=Methylenetetrahydrofolate reductase {ECO:0000256|RuleBase:RU003862};
GN ORFNames=SAMN04515677_10695 {ECO:0000313|EMBL:SDM18306.1};
OS Romboutsia lituseburensis DSM 797.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC Romboutsia.
OX NCBI_TaxID=1121325 {ECO:0000313|EMBL:SDM18306.1, ECO:0000313|Proteomes:UP000199068};
RN [1] {ECO:0000313|EMBL:SDM18306.1, ECO:0000313|Proteomes:UP000199068}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 797 {ECO:0000313|EMBL:SDM18306.1,
RC ECO:0000313|Proteomes:UP000199068};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-
CC methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH;
CC Xref=Rhea:RHEA:19821, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636,
CC ChEBI:CHEBI:18608, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.5.1.54;
CC Evidence={ECO:0000256|ARBA:ARBA00034420};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:19823;
CC Evidence={ECO:0000256|ARBA:ARBA00034420};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|RuleBase:RU003862};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|RuleBase:RU003862}.
CC -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase family.
CC {ECO:0000256|ARBA:ARBA00006743, ECO:0000256|RuleBase:RU003862}.
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DR EMBL; FNGW01000006; SDM18306.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G9R4S7; -.
DR STRING; 1121325.SAMN04515677_10695; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000199068; Unassembled WGS sequence.
DR GO; GO:0106312; F:methylenetetrahydrofolate reductase NADH activity; IEA:RHEA.
DR GO; GO:0006555; P:methionine metabolic process; IEA:InterPro.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00537; MTHFR; 1.
DR Gene3D; 3.20.20.220; -; 1.
DR InterPro; IPR029041; FAD-linked_oxidoreductase-like.
DR InterPro; IPR003171; Mehydrof_redctse-like.
DR PANTHER; PTHR45754; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR PANTHER; PTHR45754:SF3; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR Pfam; PF02219; MTHFR; 1.
DR SUPFAM; SSF51730; FAD-linked oxidoreductase; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|RuleBase:RU003862};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW ECO:0000256|RuleBase:RU003862};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU003862};
KW Reference proteome {ECO:0000313|Proteomes:UP000199068}.
SQ SEQUENCE 293 AA; 31751 MW; 69465B88FCD0D447 CRC64;
MSLLRETLES GKFAVTTEMA PPKGTDLSHL IECAKPLVGR VHAANVTDFQ SAVMRTTSLA
TCKLLKDVGL EPVIQMTGRD RNRIAIQGEM LSAGVFGINN MLALTGDHTV VGDHPQAKGV
FDLDVVGILQ TAETLMKGTD LVGNQLKGSP EFYLGASVTP EYAPIEVQLL KMRKKIAAGA
KFFQTQAVYD IEHMREFRKL TKDMDCKILA GIVPLKSPGM AKFMTANVPG IFVPDDQIER
LRAAGKENWV SEGIKMAGEF IRQLIDEDLC DGVHIMAIGA EENVPAILDA AGL
//
