ID   A0A1G9ULV9_9BURK        Unreviewed;       662 AA.
AC   A0A1G9ULV9;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 21.
DE   SubName: Full=tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein {ECO:0000313|EMBL:SDM60515.1};
GN   ORFNames=SAMN05428957_10942 {ECO:0000313|EMBL:SDM60515.1};
OS   Oryzisolibacter propanilivorax.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Oryzisolibacter.
OX   NCBI_TaxID=1527607 {ECO:0000313|EMBL:SDM60515.1, ECO:0000313|Proteomes:UP000198552};
RN   [1] {ECO:0000313|Proteomes:UP000198552}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EPL6 {ECO:0000313|Proteomes:UP000198552};
RA   Varghese N., Submissions S.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; FNHP01000009; SDM60515.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1G9ULV9; -.
DR   STRING; 1527607.SAMN05428957_10942; -.
DR   OrthoDB; 9786494at2; -.
DR   Proteomes; UP000198552; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.9.10; D-Amino Acid Oxidase, subunit A, domain 2; 1.
DR   Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR13847; SARCOSINE DEHYDROGENASE-RELATED; 1.
DR   PANTHER; PTHR13847:SF283; TRNA 5-METHYLAMINOMETHYL-2-THIOURIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN MNMC; 1.
DR   Pfam; PF01266; DAO; 1.
DR   SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
PE   4: Predicted;
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   FAD {ECO:0000256|ARBA:ARBA00022827};
KW   Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW   Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Reference proteome {ECO:0000313|Proteomes:UP000198552};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679};
KW   tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT   DOMAIN          226..627
FT                   /note="FAD dependent oxidoreductase"
FT                   /evidence="ECO:0000259|Pfam:PF01266"
SQ   SEQUENCE   662 AA;  68581 MW;  5238A60868701D73 CRC64;
     MSGGMQRPGD ALPSGWAGLA QWRVLDVGSD SGERFLAAWA AWRADPRRPR LLHWLACREA
     PQDPARLLRA MPQDSPLQPL AQALAAGMRG LLPGVHRFSF DDGRVLLTLY LGALQPLLRQ
     QQPVADAIHL HAGDAGSAQQ LADPRLCKAL ARCARRGTRL LAEGLPAQAA AALQGSGFAL
     AQAGADGACG VPALQAVFDP PWQPRAGQPP LPDVALPPGR APGECLVIGA GLAGAAVATS
     LARRGWRVTV LDAAEAPAAG ASGLPAGVFA SHVSPDDSLL SRLSRAGVRC TLQALAQLLP
     DGRGTLWDDG GVLEHDPDAP PRLAWTQGPG LDWSRPASAD ALRANGLAPD APACWHPLGG
     WVRPAQLVRR LLAEPGIRWQ GGARVERLQR ARNGAWQALD TAGALLAQGE LAVVCAGPAS
     AGVAGTPLSL QLLRGQIAWG LHGPAPAHPV AALPIEVTAS RQHAAALPCP LFPPQPVNGH
     GNLVPRVPLD LAQGAPGWVL GSTFERDQGV LPVSAADRQA GLAHNLDQLR MLLPPLARAL
     APGFAQAGTD AGVDAPADPA APCTSHTQRS ASAPVHTWAA VRCTAPDRLP LVGPVDAQAQ
     PGLWLSTAMG ARGLTLALLC GELLAARLHG EPLPLEARLA RALGSERLAE IPAKQPRGPD
     RI
//