ID A0A1H0RDK0_9BACI Unreviewed; 303 AA.
AC A0A1H0RDK0;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU362028};
DE EC=5.4.99.- {ECO:0000256|RuleBase:RU362028};
GN ORFNames=SAMN05216565_102149 {ECO:0000313|EMBL:SDP27259.1};
OS Litchfieldia salsa.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Litchfieldia.
OX NCBI_TaxID=930152 {ECO:0000313|EMBL:SDP27259.1, ECO:0000313|Proteomes:UP000199159};
RN [1] {ECO:0000313|Proteomes:UP000199159}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IBRC-M10078 {ECO:0000313|Proteomes:UP000199159};
RA Varghese N., Submissions S.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil.
CC {ECO:0000256|RuleBase:RU362028}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000256|RuleBase:RU362028};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000256|ARBA:ARBA00010876, ECO:0000256|RuleBase:RU362028}.
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DR EMBL; FNJU01000002; SDP27259.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H0RDK0; -.
DR STRING; 930152.SAMN05216565_102149; -.
DR OrthoDB; 9807829at2; -.
DR Proteomes; UP000199159; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR NCBIfam; TIGR00005; rluA_subfam; 1.
DR PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR PANTHER; PTHR21600:SF44; PSEUDOU_SYNTH_2 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|RuleBase:RU362028};
KW Reference proteome {ECO:0000313|Proteomes:UP000199159};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 15..75
FT /note="RNA-binding S4"
FT /evidence="ECO:0000259|SMART:SM00363"
FT ACT_SITE 138
FT /evidence="ECO:0000256|PIRSR:PIRSR606225-1"
SQ SEQUENCE 303 AA; 33984 MW; DD988FB2844C99F2 CRC64;
MNENMIIVEN EELSDRIDKV ITAQNSDWSR SQVQQWIKEG NVTVNGQKIK SNYKSNPGDK
IEITIPSPEL LDVIPEEMNL EIFYEDSDVL VVNKPKGMVV HPAPGHYSGT LVNGLMAHCK
DLSGINGVLR PGIVHRIDKD TSGLLMVAKN DLAHESLVQQ LVDKTVTRKY KAIVHGNIPH
DQGTIDAPIG RDKRDRQSMT VTDDNSKEAV THFNVLERLK DFTYIECQLE TGRTHQIRVH
MKYIGYPLAG DPKYGPKKTL SIGGQALHAG ILGFIHPRTK EYLEFEAPLP SEFEALLEEL
KSN
//
