ID   A0A1H0S031_9BACI        Unreviewed;       301 AA.
AC   A0A1H0S031;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   24-JAN-2024, entry version 18.
DE   RecName: Full=Non-homologous end joining protein Ku {ECO:0000256|HAMAP-Rule:MF_01875};
GN   Name=ku {ECO:0000256|HAMAP-Rule:MF_01875};
GN   ORFNames=SAMN05216565_102478 {ECO:0000313|EMBL:SDP35201.1};
OS   Litchfieldia salsa.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Litchfieldia.
OX   NCBI_TaxID=930152 {ECO:0000313|EMBL:SDP35201.1, ECO:0000313|Proteomes:UP000199159};
RN   [1] {ECO:0000313|Proteomes:UP000199159}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IBRC-M10078 {ECO:0000313|Proteomes:UP000199159};
RA   Varghese N., Submissions S.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: With LigD forms a non-homologous end joining (NHEJ) DNA
CC       repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds
CC       linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA
CC       nor ssDNA. Recruits and stimulates the ligase activity of LigD.
CC       {ECO:0000256|HAMAP-Rule:MF_01875}.
CC   -!- SUBUNIT: Homodimer. Interacts with LigD. {ECO:0000256|HAMAP-
CC       Rule:MF_01875}.
CC   -!- SIMILARITY: Belongs to the prokaryotic Ku family. {ECO:0000256|HAMAP-
CC       Rule:MF_01875}.
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DR   EMBL; FNJU01000002; SDP35201.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1H0S031; -.
DR   STRING; 930152.SAMN05216565_102478; -.
DR   OrthoDB; 9795084at2; -.
DR   Proteomes; UP000199159; Unassembled WGS sequence.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:UniProtKB-UniRule.
DR   CDD; cd00789; KU_like; 1.
DR   Gene3D; 2.40.290.10; -; 1.
DR   HAMAP; MF_01875; Prokaryotic_Ku; 1.
DR   InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR   InterPro; IPR009187; Prok_Ku.
DR   InterPro; IPR016194; SPOC-like_C_dom_sf.
DR   NCBIfam; TIGR02772; Ku_bact; 1.
DR   PANTHER; PTHR41251; NON-HOMOLOGOUS END JOINING PROTEIN KU; 1.
DR   PANTHER; PTHR41251:SF1; NON-HOMOLOGOUS END JOINING PROTEIN KU; 1.
DR   Pfam; PF02735; Ku; 1.
DR   PIRSF; PIRSF006493; Prok_Ku; 1.
DR   SMART; SM00559; Ku78; 1.
DR   SUPFAM; SSF100939; SPOC domain-like; 1.
PE   3: Inferred from homology;
KW   DNA damage {ECO:0000256|HAMAP-Rule:MF_01875};
KW   DNA recombination {ECO:0000256|HAMAP-Rule:MF_01875};
KW   DNA repair {ECO:0000256|HAMAP-Rule:MF_01875};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW   Rule:MF_01875}; Reference proteome {ECO:0000313|Proteomes:UP000199159}.
FT   DOMAIN          52..181
FT                   /note="Ku"
FT                   /evidence="ECO:0000259|SMART:SM00559"
FT   REGION          256..301
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        263..301
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   301 AA;  33994 MW;  D141968E320D28FB CRC64;
     MHTMWKGSIS FGLVNIPIKL FAATEDKDVK LRNLHKECHT PIQYEKVCSH CDKDVTNEDI
     VKGYEYVKGK FIILTDEELK ELKDEHEDKS VEIVDFVNLE DIDPIYFSRS YFMGPGDNGS
     KAYGLLREAL KTSGKIGIAE ITIRSKQQMA IIRVYKNSLV METIHYPDEV RNVDEVPAVP
     ENVEINEKEL ETAIMLIDQL TTDFQPEKYQ DDYRTALLDL IQTKVNNNEG TTSKDTAPRT
     NVVDLMSALQ ASIERTKGKE TTAVAPKKTT AQQKTQTSVS EAEPKAGTTQ KVRTTRKKVG
     S
//