ID   A0A1H0VL48_9CLOT        Unreviewed;       809 AA.
AC   A0A1H0VL48;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 18.
DE   SubName: Full=Glycerol dehydratase, cobalamin-independent, large subunit {ECO:0000313|EMBL:SDP78776.1};
GN   ORFNames=SAMN04488529_11735 {ECO:0000313|EMBL:SDP78776.1};
OS   Clostridium gasigenes.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=94869 {ECO:0000313|EMBL:SDP78776.1, ECO:0000313|Proteomes:UP000198597};
RN   [1] {ECO:0000313|EMBL:SDP78776.1, ECO:0000313|Proteomes:UP000198597}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 12272 {ECO:0000313|EMBL:SDP78776.1,
RC   ECO:0000313|Proteomes:UP000198597};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; FNJM01000017; SDP78776.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1H0VL48; -.
DR   STRING; 94869.SAMN04488529_11735; -.
DR   OrthoDB; 9803969at2; -.
DR   Proteomes; UP000198597; Unassembled WGS sequence.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   CDD; cd01677; PFL2_DhaB_BssA; 1.
DR   Gene3D; 3.20.70.20; -; 1.
DR   InterPro; IPR019777; Form_AcTrfase_GR_CS.
DR   InterPro; IPR001150; Gly_radical.
DR   InterPro; IPR010098; PFL2/GDeHydtase_fam.
DR   InterPro; IPR004184; PFL_dom.
DR   NCBIfam; TIGR01774; PFL2-3; 1.
DR   PANTHER; PTHR43641:SF2; DEHYDRATASE YBIW-RELATED; 1.
DR   PANTHER; PTHR43641; FORMATE ACETYLTRANSFERASE 3-RELATED; 1.
DR   Pfam; PF01228; Gly_radical; 1.
DR   Pfam; PF02901; PFL-like; 1.
DR   SUPFAM; SSF51998; PFL-like glycyl radical enzymes; 1.
DR   PROSITE; PS00850; GLY_RADICAL_1; 1.
DR   PROSITE; PS51149; GLY_RADICAL_2; 1.
DR   PROSITE; PS51554; PFL; 1.
PE   4: Predicted;
KW   Lyase {ECO:0000256|ARBA:ARBA00023239};
KW   Organic radical {ECO:0000256|ARBA:ARBA00022818, ECO:0000256|PROSITE-
KW   ProRule:PRU00493}; Reference proteome {ECO:0000313|Proteomes:UP000198597}.
FT   DOMAIN          18..682
FT                   /note="PFL"
FT                   /evidence="ECO:0000259|PROSITE:PS51554"
FT   DOMAIN          689..809
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000259|PROSITE:PS51149"
FT   MOD_RES         785
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00493"
SQ   SEQUENCE   809 AA;  91172 MW;  7E642A6F7C103195 CRC64;
     METLKRNFLS KGFKNSTERV YKLKEEILSA QPCVEVERAR LITESFKETE DQPIIIRKAK
     ALEKILNEIP VVIRDGELIV GTLSKNPRSA QVFPEFSNKW LVSEFDRIGK RTSDKYEISE
     DVKSELVELF KYWDGKTVSE LAKAYMPQET QDAMSTNVFT CGNYYFNGLG HYVVDYEKVI
     KNGLKGIVEE VQNKINNSNK ADPEFIKNRE FWESIIISCN AAINFAKRYS KLALNMAEDE
     NNSTRKRELI NISEICDRAL GNGSDTFYEA VQSFWFIQVI ISLESNGHAI SPGRFDQYTY
     EYFRKDIDSG NIDMLEAQEL IDCLWVKFND ITKIRDEVVT KAFSGYGMFQ NLIVGGQTVD
     GNDATNELSY MCIEATGSLK LPQPSLSARI WSKTPEEFLL RCCELVRLGL GFPALYNDEV
     IIPSLLNRGV TLEDSRNYSI VGCVEPQCGG KTDGWYDAAF FNLAKVLEMT VNNGKFNEKQ
     IGPKTGEFTS FKNLDEFIKA YKEQTQYFVS QMVCADNCVD MAHRERAPLP FVSCMMDDCI
     ERGKSIQDGG AHYRYSGPLG VGIANIGDSF MAIKKLVFED KKITLNDLKD ALLTNFGQDE
     KCDEFKRNKF NEIKNILIHK APKFGNDLDE VDELTREGAL IYCKEVEKHT NVRGGKFLPG
     LYPVSNNVHL GSQVGATPDG RSANQPLADG VSPNRGMDTN GPTAAANSVA KLDHFIAASG
     TLFNQKFNPS SLKNDNGLKN LASLIRGYFD EKGMHVQFNV IDKAVLIEAQ KHPEQYRDLV
     IRVAGYSAQF ICLDKSIQDD IIARTEQAF
//