ID A0A1H0YBQ2_9FLAO Unreviewed; 948 AA.
AC A0A1H0YBQ2;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=SAMN05421664_0623 {ECO:0000313|EMBL:SDQ12557.1};
OS Chryseobacterium soldanellicola.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales; Weeksellaceae;
OC Chryseobacterium group; Chryseobacterium.
OX NCBI_TaxID=311333 {ECO:0000313|EMBL:SDQ12557.1, ECO:0000313|Proteomes:UP000199627};
RN [1] {ECO:0000313|EMBL:SDQ12557.1, ECO:0000313|Proteomes:UP000199627}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17072 {ECO:0000313|EMBL:SDQ12557.1,
RC ECO:0000313|Proteomes:UP000199627};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
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DR EMBL; FNKL01000001; SDQ12557.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H0YBQ2; -.
DR STRING; 311333.SAMN05421664_0623; -.
DR Proteomes; UP000199627; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 10..271
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 357..538
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 706..912
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 948 AA; 107615 MW; 3A52C72B3A2CD0F5 CRC64;
MENMDATQDK RLFLIDAYAM IFRGYHAMYK SQRYTNSGME TTAIFGFMNS LIELIRKEKP
THLAVVFDVG QASVRTNDYA DYKANRSETP EAIKTGVPYI HRILEAMHVP RLGIEGYEAD
DVIGTIACKA EKEGYTTFMV TPDKDFGQLV TDKIKIYKPG YKGGDVQILG VEEINAKYEI
EDPKQVIDYL AMMGDASDNI PGLEGVGEKT AMKFLKEFGS IENLLANTDK LKGKLKEKVE
ASAERGILSK KLATIICDVP IEFHQEQYDL ETPDFEKVKE IFDEIEFRRL YENLYRAFAP
STTETTVVTE VEVTISTETA QQKVAQAVGQ LDLFATYEEL DQATSTKSTI EHNDHLYQFI
DNPKAQKILV QNLLKQKVVC FDTETTSLNE LEAELVGMSF SYKKGLAYYI PLSEDQGEVL
QTLEIFRPFF EKEDLLKVAH NLKFDYKILK QYDITVKGAM FDTMIAHYLL NPDGRHGMDY
LSEVYLNYKP VSIETIIGKK GKNQGTFRDA DLRTQTDYAA EDADITFQLY ELFAPQLKKE
NLEDLFFNIE MPLMEVLAKM ELAGISLDEK WLAQESIDLE NDLRQLESKI FEFSGEEFNM
NSPKQLGEVL FEKMQLDPKA KKTKTGQYAT SEDVLQKLAS KHEIIKHILE YRTYQKLKST
YVDALPSQID KDERVHTNFS QTTAATGRLA SVNPNLQNIP IRTLRGQQIR GAFVSAEGKK
IISADYSQIE LRLIAEISGE ENMIKAFQNG EDIHASTAAK LFKIPLEEVS KTQRSQAKTV
NFGIIYGQGA FALAEQTGLS RTEAKQMIEA YFETYPKLKE YMAEQVNKAR QLGYVETILG
RKRHLKDINS NNFVVRGHAE RNAVNAPVQG SAADVVKMAM IKIQKELEKE KLETKMLLQV
HDELVFESPI NEIELATNII KIEMESAIET QVPLLVEVGV GNNWLEAH
//
