ID   A0A1H1H9S0_9ACTN        Unreviewed;       274 AA.
AC   A0A1H1H9S0;
DT   12-APR-2017, integrated into UniProtKB/TrEMBL.
DT   12-APR-2017, sequence version 1.
DT   27-MAR-2024, entry version 28.
DE   SubName: Full=2,5-diketo-D-gluconate reductase A {ECO:0000313|EMBL:SDR22255.1};
GN   ORFNames=SAMN04489765_3958 {ECO:0000313|EMBL:SDR22255.1};
OS   Tsukamurella pulmonis.
OC   Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Tsukamurellaceae;
OC   Tsukamurella.
OX   NCBI_TaxID=47312 {ECO:0000313|EMBL:SDR22255.1, ECO:0000313|Proteomes:UP000183053};
RN   [1] {ECO:0000313|Proteomes:UP000183053}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 44142 {ECO:0000313|Proteomes:UP000183053};
RA   Varghese N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; FNLF01000002; SDR22255.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1H1H9S0; -.
DR   STRING; 47312.SAMN04489765_3958; -.
DR   Proteomes; UP000183053; Unassembled WGS sequence.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR   GO; GO:0044281; P:small molecule metabolic process; IEA:UniProt.
DR   Gene3D; 3.20.20.100; NADP-dependent oxidoreductase domain; 1.
DR   InterPro; IPR020471; AKR.
DR   InterPro; IPR018170; Aldo/ket_reductase_CS.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   PANTHER; PTHR43827; 2,5-DIKETO-D-GLUCONIC ACID REDUCTASE; 1.
DR   PANTHER; PTHR43827:SF3; ALDO-KETO REDUCTASE; 1.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   PIRSF; PIRSF000097; AKR; 2.
DR   PRINTS; PR00069; ALDKETRDTASE.
DR   SUPFAM; SSF51430; NAD(P)-linked oxidoreductase; 1.
DR   PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR   PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE   4: Predicted;
KW   NADP {ECO:0000256|ARBA:ARBA00022857}.
FT   DOMAIN          17..258
FT                   /note="NADP-dependent oxidoreductase"
FT                   /evidence="ECO:0000259|Pfam:PF00248"
FT   ACT_SITE        50
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000097-1"
FT   BINDING         108
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000097-2"
FT   SITE            75
FT                   /note="Lowers pKa of active site Tyr"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000097-3"
SQ   SEQUENCE   274 AA;  30131 MW;  6EE8980F98BBFB91 CRC64;
     MTVPNVKLNN GTPIPQLGFG VWQVPDDGAR DAVAEALRVG YRSIDTAKVY ENEAGTGRAI
     AESGLARDEV FLTTKLWNSD QGFDETLRAF DGSLERLGTD YVDLYLIHWP VLEYDRYVDT
     FKAFQRIRDE GRAKAIGVSN FTVEALRRVI DETGETPTVN QIELHPGLPQ DELRAFHAEH
     GIATEAWSPL GQGESLKDPV ITEVAAAHGV TPAQAILRWH LQLGNVVIPK SVTPARIAEN
     FDVFGFELSA DEVARISAVP GQRIGPDPAT FNLR
//