ID A0A1H1N1P4_9CELL Unreviewed; 1212 AA.
AC A0A1H1N1P4;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Replicative DNA helicase {ECO:0000256|ARBA:ARBA00021957, ECO:0000256|RuleBase:RU362085};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551, ECO:0000256|RuleBase:RU362085};
GN ORFNames=SAMN04489860_0407 {ECO:0000313|EMBL:SDR92916.1};
OS Paraoerskovia marina.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Cellulomonadaceae;
OC Paraoerskovia.
OX NCBI_TaxID=545619 {ECO:0000313|EMBL:SDR92916.1, ECO:0000313|Proteomes:UP000185663};
RN [1] {ECO:0000313|EMBL:SDR92916.1, ECO:0000313|Proteomes:UP000185663}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 22126 {ECO:0000313|EMBL:SDR92916.1,
RC ECO:0000313|Proteomes:UP000185663};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Participates in initiation and elongation during chromosome
CC replication; it exhibits DNA-dependent ATPase activity.
CC {ECO:0000256|ARBA:ARBA00002835, ECO:0000256|RuleBase:RU362085}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665,
CC ECO:0000256|RuleBase:RU362085};
CC -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC {ECO:0000256|ARBA:ARBA00008428, ECO:0000256|RuleBase:RU362085}.
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DR EMBL; LT629776; SDR92916.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H1N1P4; -.
DR STRING; 545619.SAMN04489860_0407; -.
DR eggNOG; COG0305; Bacteria.
DR OrthoDB; 9773982at2; -.
DR Proteomes; UP000185663; Chromosome i.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-UniRule.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd00081; Hint; 1.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 2.
DR Gene3D; 3.10.28.10; Homing endonucleases; 2.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR InterPro; IPR007692; DNA_helicase_DnaB.
DR InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR InterPro; IPR016136; DNA_helicase_N/primase_C.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR00665; DnaB; 1.
DR NCBIfam; TIGR01443; intein_Cterm; 1.
DR NCBIfam; TIGR01445; intein_Nterm; 1.
DR PANTHER; PTHR30153:SF2; REPLICATIVE DNA HELICASE; 1.
DR PANTHER; PTHR30153; REPLICATIVE DNA HELICASE DNAB; 1.
DR Pfam; PF00772; DnaB; 1.
DR Pfam; PF03796; DnaB_C; 3.
DR Pfam; PF14890; Intein_splicing; 1.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 2.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 2.
DR SUPFAM; SSF55608; Homing endonucleases; 1.
DR SUPFAM; SSF48024; N-terminal domain of DnaB helicase; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
DR PROSITE; PS51199; SF4_HELICASE; 3.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU362085};
KW Autocatalytic cleavage {ECO:0000256|ARBA:ARBA00022813};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU362085};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU362085};
KW Helicase {ECO:0000256|RuleBase:RU362085, ECO:0000313|EMBL:SDR92916.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362085};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU362085}; Primosome {ECO:0000256|RuleBase:RU362085};
KW Protein splicing {ECO:0000256|ARBA:ARBA00023000};
KW Reference proteome {ECO:0000313|Proteomes:UP000185663};
KW Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT DOMAIN 195..232
FT /note="SF4 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51199"
FT DOMAIN 358..548
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000259|PROSITE:PS50819"
FT DOMAIN 639..805
FT /note="SF4 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51199"
FT DOMAIN 1132..1154
FT /note="Intein C-terminal splicing"
FT /evidence="ECO:0000259|PROSITE:PS50818"
FT DOMAIN 1154..1212
FT /note="SF4 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51199"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1212 AA; 132236 MW; D16CF3F72522B40E CRC64;
MSIEELEAGF GGEGPSGGAA FDRTPPQDVA AEQSVLGGML ISKDAIADVI EQVRGTDFYR
PAHEAVYDAV VDLYGRGEPA DAITVVAELQ KRGELGRIGG APYIHDLISM VPTAANAGYY
ARIVRERAVL RRLVEAGTRI VQLGYAADGG DVDTIVNNAQ AEVYSVTERR AAEDYVPLGD
IIGGTVDEIE AAGHRGEGMV GVPTGFADLD RLTNGLHPGQ MIVLAARPAI GKALALDTPL
PTPSGWTTMG EVQVGDELIA ADGTPTQVIR ATEVLEDRPC YEVVFDDGTT IVADALHEWA
TTTRAERRHR GSRGGIRTTD EIAATVRCDT ADRRANHAIR TSAALELAEA ELPLHPYALG
VWLGDGHAAG ARFTSADPEI PMRIEGLGLV ATQQGNSDGA AQLYSLRLPP APAIEERPCV
VCGAVFLPRT SQVRTCGRSC GGRAKAVSSP VPEPTCPDCG RESTGLRRCQ SCHNAHGTVQ
AILRSLDVMS NKHIPEQYLR ASESQRRELL AGLLDTDGTV NPTGSPQFCV TSRRLALDVR
ELVHSLGYRS GWSERAVRGR TVGTSTAYTI TFTTDDDVFA LERKRLLHKE RRRPSTPRLT
SRFVVDVREV PSVPVRCVEI AHPEHLYLAS RSMIPTHNST LGIDIARSAS IKHDMTSVVF
SLEMSRNEIT MRLLSAEARI HLQKMRNGSM GEDDWQKLAS TMGKITEAPL FIDDSPNMSL
MEIRAKCRRL KQKHDLRLVV IDYLQLMTSG KRVESRQQEV SEFSRALKLL AKELEVPLIA
ISQLNRGAEQ RTDKKPAMSD LRESGCLTED TRVLRADTGA ETTMGELFAL GAKDVPVWAL
NERLQYVRRH LTHVFPTGVK QVYRLRLSSG KEISATANHP FLTYDGWRPL GELEVGSRLG
VPRHVPAPEQ EAEWDDRKVA ALANLVSGPM KGSTTPETLG DDVWLDREVG VPAAVFQLPK
RQIALFLHRI ASLCGRVVLH ESGTGVVQIV GTVRSLDGIA RLLLRFGISA QSRDVARGSA
LEIRGTDDLR RFLQEIGIER DERSADELLL RVREAAGDLN SGPLTVWDEV RHILEADEPT
TRAGVQVVPR GESRTTLGAV AGVLERADLE IEAVNDLAWE EIVAVEPLGE QAVFDATVLD
SHNFIANGIA VHNSIEQDAD MVILLHREDA YEKESPRAGE ADLIVAKHRN GPTDTITVAF
QGHYSRFVDM QA
//
