ID   A0A1H1PYZ2_9MICO        Unreviewed;       536 AA.
AC   A0A1H1PYZ2;
DT   18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT   18-JAN-2017, sequence version 1.
DT   27-MAR-2024, entry version 22.
DE   SubName: Full=Assimilatory nitrate reductase (NADH) beta subunit {ECO:0000313|EMBL:SDS16442.1};
GN   ORFNames=SAMN04489721_0874 {ECO:0000313|EMBL:SDS16442.1};
OS   Agromyces flavus.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Agromyces.
OX   NCBI_TaxID=589382 {ECO:0000313|EMBL:SDS16442.1, ECO:0000313|Proteomes:UP000199482};
RN   [1] {ECO:0000313|Proteomes:UP000199482}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CPCC 202695 {ECO:0000313|Proteomes:UP000199482};
RA   Varghese N., Submissions S.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   -!- COFACTOR:
CC       Name=siroheme; Xref=ChEBI:CHEBI:60052;
CC         Evidence={ECO:0000256|ARBA:ARBA00001929};
CC   -!- PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
CC       {ECO:0000256|ARBA:ARBA00005096}.
CC   -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S domain
CC       family. {ECO:0000256|ARBA:ARBA00010429}.
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DR   EMBL; LT629755; SDS16442.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1H1PYZ2; -.
DR   STRING; 589382.SAMN04489721_0874; -.
DR   OrthoDB; 1145at2; -.
DR   Proteomes; UP000199482; Chromosome i.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR   Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR   InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR   InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR023753; FAD/NAD-binding_dom.
DR   PANTHER; PTHR43809; NITRITE REDUCTASE (NADH) LARGE SUBUNIT; 1.
DR   PANTHER; PTHR43809:SF1; NITRITE REDUCTASE (NADH) LARGE SUBUNIT; 1.
DR   Pfam; PF04324; Fer2_BFD; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   PRINTS; PR00368; FADPNR.
DR   SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 2.
PE   3: Inferred from homology;
KW   Heme {ECO:0000256|ARBA:ARBA00022617}; Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT   DOMAIN          6..316
FT                   /note="FAD/NAD(P)-binding"
FT                   /evidence="ECO:0000259|Pfam:PF07992"
FT   DOMAIN          475..524
FT                   /note="BFD-like [2Fe-2S]-binding"
FT                   /evidence="ECO:0000259|Pfam:PF04324"
SQ   SEQUENCE   536 AA;  56473 MW;  CB1B03CC4976C2A0 CRC64;
     MTEPLRIVLI GYGPVGARFV EELLPEVAAR RIVVTVVGGE HAEAYNRVLV ADYAVGNTEL
     DAMLVGDREA AQAAGARVLL GVAATSIDRS ARVVRCSNGE ALPYDRVVLA TGSRANVPTL
     DGVERHRRDL ASLEKHASTL VARDDALPAG ITALRDLDDA QRVLEAVRGR RRVIVLGAGV
     LGLELALAAQ HAGAQVCVVH HGPIPMPRNL DRGSGQVLRA ALRRTGITVI AHSRAEAVGF
     RTDEDGTRRF DMLVTADGKQ LRGDLLVLSC GVSPRNELAT LAGLRTATGV VVGPDLRSWT
     DPDVYAIGDC AQVVERTDET AGRRDLPGAP SGLIGPGWRQ ASWLAAALAA EVAGIGTEAA
     LPTEREPIVM LKAEHVDVVA VGDVSADPWD DDALAPRRRV AQWADPEHLR YVKMVTEDGV
     LTGFASVGMP RTAAELTLLF ERGGELPADR SLLLRFDGPD YDPAGDADAF APETTVCWCN
     GVTVGRIEES AACGHGTVEC VGRDTRAGTG CGGCRGRIAE VLARTAQADG APSLTA
//