ID   A0A1H2ASJ6_9ACTN        Unreviewed;       665 AA.
AC   A0A1H2ASJ6;
DT   18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT   18-JAN-2017, sequence version 1.
DT   24-JAN-2024, entry version 20.
DE   RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE            Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE            EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN   ORFNames=SAMN04489716_4070 {ECO:0000313|EMBL:SDT48980.1};
OS   Actinoplanes derwentensis.
OC   Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC   Micromonosporaceae; Actinoplanes.
OX   NCBI_TaxID=113562 {ECO:0000313|EMBL:SDT48980.1, ECO:0000313|Proteomes:UP000198688};
RN   [1] {ECO:0000313|EMBL:SDT48980.1, ECO:0000313|Proteomes:UP000198688}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 43941 {ECO:0000313|EMBL:SDT48980.1,
RC   ECO:0000313|Proteomes:UP000198688};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC         Evidence={ECO:0000256|ARBA:ARBA00001412,
CC         ECO:0000256|PIRNR:PIRNR001084};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC       {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
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DR   EMBL; LT629758; SDT48980.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1H2ASJ6; -.
DR   STRING; 113562.SAMN04489716_4070; -.
DR   OrthoDB; 9800974at2; -.
DR   Proteomes; UP000198688; Chromosome i.
DR   GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR013738; Beta_galactosidase_Trimer.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR003476; Glyco_hydro_42.
DR   InterPro; IPR013529; Glyco_hydro_42_N.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR   PANTHER; PTHR36447:SF1; BETA-GALACTOSIDASE GANA; 1.
DR   Pfam; PF02449; Glyco_hydro_42; 1.
DR   Pfam; PF08532; Glyco_hydro_42M; 1.
DR   PIRSF; PIRSF001084; B-galactosidase; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR001084};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR001084};
KW   Reference proteome {ECO:0000313|Proteomes:UP000198688}.
FT   DOMAIN          25..395
FT                   /note="Glycoside hydrolase family 42 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF02449"
FT   DOMAIN          407..610
FT                   /note="Beta-galactosidase trimerisation"
FT                   /evidence="ECO:0000259|Pfam:PF08532"
FT   ACT_SITE        161
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT   ACT_SITE        318
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT   BINDING         122
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT   BINDING         160
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT   BINDING         326
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ   SEQUENCE   665 AA;  74581 MW;  5D352334E6D31A23 CRC64;
     MTQSFETPRR WLRRAGAARL EFGADYNPDQ WPREVWDDDV RAMRQAGVTI VSLAIFSWAR
     LEPREGVFDF AWLDEVMDLL HAGGITVDLA TATASPPPWL TRKHPEILPV TRTGETLWPG
     ARQHWRPTSP VFREHALRLV RELAVRYAEH PALVAWHVNN ELGCHNIYDY SDDAARAFRL
     WLRERYNTVE ALNHAWGTAF WAQRYDDFEE VLPPRLAASH PNPTQQLDFK RFSSDALKEH
     LRAERAVLRR ITPEVPVTTN FMVMGETKGM NYADWAAEVD FVSNDHYVLP GPQARDELSF
     SANLTGNLAG GHPWFLMEHS TSAVNWQPVN VAKKPGELAR DSLTHVAHGA DAVCYFQWRQ
     SKAGAEKYHS AMLSHSGTDS DLFRDVVRLG ERLRELAPIA GSRRRRAPVG IVFDWESWWV
     SEHDSHPTDR LRYRPEALDW YTALLDAGIR ADVVPPDAPL EEYRVLIAPI LHVVPGALAT
     RLRRYAAGGG HLVATYFSGI VDEHDHVWLG GYPGALREVL GIRIEEFGPL LDHESVELDN
     GTTGTLWTDR IDVTGTDVEV LAHYKTGDQA ERAAVTRRAV GTGSASYVST RLGPAGLAPL
     LPALLGPAGV SSELPAELAG RVELTIRGDH WFLINRTDSP VELDSLGEPV LEPRGVRVLH
     RDSRV
//