ID   A0A1H2B393_9ACTN        Unreviewed;       547 AA.
AC   A0A1H2B393;
DT   18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT   18-JAN-2017, sequence version 1.
DT   27-MAR-2024, entry version 23.
DE   SubName: Full=3-oxosteroid 1-dehydrogenase {ECO:0000313|EMBL:SDT52653.1};
GN   ORFNames=SAMN04489716_4302 {ECO:0000313|EMBL:SDT52653.1};
OS   Actinoplanes derwentensis.
OC   Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC   Micromonosporaceae; Actinoplanes.
OX   NCBI_TaxID=113562 {ECO:0000313|EMBL:SDT52653.1, ECO:0000313|Proteomes:UP000198688};
RN   [1] {ECO:0000313|EMBL:SDT52653.1, ECO:0000313|Proteomes:UP000198688}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 43941 {ECO:0000313|EMBL:SDT52653.1,
RC   ECO:0000313|Proteomes:UP000198688};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974};
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DR   EMBL; LT629758; SDT52653.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1H2B393; -.
DR   STRING; 113562.SAMN04489716_4302; -.
DR   OrthoDB; 9813348at2; -.
DR   Proteomes; UP000198688; Chromosome i.
DR   GO; GO:0033765; F:steroid dehydrogenase activity, acting on the CH-CH group of donors; IEA:UniProt.
DR   GO; GO:0008202; P:steroid metabolic process; IEA:UniProt.
DR   Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR   InterPro; IPR003953; FAD-binding_2.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR027477; Succ_DH/fumarate_Rdtase_cat_sf.
DR   PANTHER; PTHR43400:SF7; FAD_BINDING_2 DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR43400; FUMARATE REDUCTASE; 1.
DR   Pfam; PF00890; FAD_binding_2; 1.
DR   SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR   SUPFAM; SSF56425; Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain; 1.
PE   4: Predicted;
KW   Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Reference proteome {ECO:0000313|Proteomes:UP000198688}.
FT   DOMAIN          8..529
FT                   /note="FAD-dependent oxidoreductase 2 FAD binding"
FT                   /evidence="ECO:0000259|Pfam:PF00890"
SQ   SEQUENCE   547 AA;  58137 MW;  948E98CFE69A75C1 CRC64;
     MAVTVEYDVV VVGAGAAGMT AALTTARSGL NTVLVEKAAV FGGSTARSGA GIWVPNNEVL
     LAAGVPDTFD KADTYLAAVV GADVPVARRR AYLTYGPRTI SYLLRWTPLR FAWMEGYPDY
     YPELSGGMPN GRSVEPQAFD ANILGDEQAR LNPAYQASPA GTVVYTVDYK WLSLIARHPK
     GLATATELVW RYLQLSAQGQ KPVTMGQALA GGLRAGLIAA GVPVWLNTPL LDLYVANGRV
     AGVLARKDGV ETLIRARRGV VMASGGFEHN ERMRKQYQAA PIGVDWTIGA DSNTGDGIEA
     GQRAGAALGL MDDAWWGPMI PLPGEPWFCL SERTLPGTIF VNQAGQRFVN EAAPYSDVVH
     TMYAKGHVPC WMITDQDYRN RYLFKDLAPM LPYPDDWYSS GALVKDWTIA GLAAKIGVPA
     AALNATISRF NGQARAGRDP DQHRGDSVYD AYFADPTNLP SPCLAPIWLP PYYAFKIVPG
     DLGTKGGLLT DARARVVKPD GSVVPGLWAA GNASSAVMGH SYAGAGSTLG PAMTFGHVAG
     LDIAAAS
//