ID A0A1H2WK67_9FIRM Unreviewed; 419 AA.
AC A0A1H2WK67;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 17.
DE RecName: Full=histidine--tRNA ligase {ECO:0000256|ARBA:ARBA00012815};
DE EC=6.1.1.21 {ECO:0000256|ARBA:ARBA00012815};
GN ORFNames=SAMN05216391_11837 {ECO:0000313|EMBL:SDW80951.1};
OS Lachnospiraceae bacterium KHCPX20.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX NCBI_TaxID=1855375 {ECO:0000313|EMBL:SDW80951.1, ECO:0000313|Proteomes:UP000199590};
RN [1] {ECO:0000313|EMBL:SDW80951.1, ECO:0000313|Proteomes:UP000199590}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KHCPX20 {ECO:0000313|EMBL:SDW80951.1,
RC ECO:0000313|Proteomes:UP000199590};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-histidine + tRNA(His) = AMP + diphosphate + H(+) + L-
CC histidyl-tRNA(His); Xref=Rhea:RHEA:17313, Rhea:RHEA-COMP:9665,
CC Rhea:RHEA-COMP:9689, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57595, ChEBI:CHEBI:78442,
CC ChEBI:CHEBI:78527, ChEBI:CHEBI:456215; EC=6.1.1.21;
CC Evidence={ECO:0000256|ARBA:ARBA00001137};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00008226}.
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DR EMBL; FNOC01000018; SDW80951.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H2WK67; -.
DR STRING; 1855375.SAMN05216391_11837; -.
DR Proteomes; UP000199590; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0004821; F:histidine-tRNA ligase activity; IEA:InterPro.
DR GO; GO:0016740; F:transferase activity; IEA:UniProt.
DR GO; GO:0006427; P:histidyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd00773; HisRS-like_core; 1.
DR InterPro; IPR006195; aa-tRNA-synth_II.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR015807; His-tRNA-ligase.
DR InterPro; IPR041715; HisRS-like_core.
DR InterPro; IPR004516; HisRS/HisZ.
DR NCBIfam; TIGR00442; hisS; 1.
DR PANTHER; PTHR11476:SF7; HISTIDINE--TRNA LIGASE; 1.
DR PANTHER; PTHR11476; HISTIDYL-TRNA SYNTHETASE; 1.
DR Pfam; PF13393; tRNA-synt_His; 1.
DR PIRSF; PIRSF001549; His-tRNA_synth; 1.
DR SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000313|EMBL:SDW80951.1};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Ligase {ECO:0000313|EMBL:SDW80951.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000199590}.
FT DOMAIN 10..353
FT /note="Aminoacyl-transfer RNA synthetases class-II family
FT profile"
FT /evidence="ECO:0000259|PROSITE:PS50862"
SQ SEQUENCE 419 AA; 47295 MW; 623962CC69DD9390 CRC64;
MALKKKPVNG MKDILPREME IRDYVTGVIK ETYRSFGFTP IETPCMEDIA NLTSNQGGEN
EKLIFKVLKR GEKLHLDTAK TDLDVSDMGL RYDLTLPLSR FYANHENDLS SPFKALQIGS
VWRADRPQRG RYRQFTQCDI DILGEPSNLA EIELITATTT CLGRLNFKNF EIRINERRIL
KAMAAYAGFE EKDYDGIFIT LDKMDKIGLE GVEAELMEDG FAEESVKKYT EIFEALSDKK
DVAEGVSYIK EKLGDFLEQE VVDSLTDIAS AVNANKAAEF ELVFDPTLVR GMGYYTGTIF
EIAIPEFGGS CGGGGRYDRM IGKFAGHDVP ACGFSIGFER IILLLMEQDF KIPTAPKKTA
YLVEKKYPAD QLAKVMQQAA KARANGQTVL VVRMNKNKKF QKEQLSKDGY EEFVEFYNN
//
