ID   A0A1H3F3G0_9PSEU        Unreviewed;       377 AA.
AC   A0A1H3F3G0;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   24-JAN-2024, entry version 14.
DE   SubName: Full=Glutathione-independent formaldehyde dehydrogenase {ECO:0000313|EMBL:SDX84724.1};
GN   ORFNames=SAMN05216215_101623 {ECO:0000313|EMBL:SDX84724.1};
OS   Saccharopolyspora shandongensis.
OC   Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC   Pseudonocardiaceae; Saccharopolyspora.
OX   NCBI_TaxID=418495 {ECO:0000313|EMBL:SDX84724.1, ECO:0000313|Proteomes:UP000199529};
RN   [1] {ECO:0000313|EMBL:SDX84724.1, ECO:0000313|Proteomes:UP000199529}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CGMCC 4.3530 {ECO:0000313|EMBL:SDX84724.1,
RC   ECO:0000313|Proteomes:UP000199529};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|ARBA:ARBA00001947,
CC         ECO:0000256|RuleBase:RU361277};
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family. {ECO:0000256|RuleBase:RU361277}.
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DR   EMBL; FNOK01000016; SDX84724.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1H3F3G0; -.
DR   STRING; 418495.SAMN05216215_101623; -.
DR   OrthoDB; 241504at2; -.
DR   Proteomes; UP000199529; Unassembled WGS sequence.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd08282; PFDH_like; 1.
DR   Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR013149; ADH-like_C.
DR   InterPro; IPR013154; ADH-like_N.
DR   InterPro; IPR002328; ADH_Zn_CS.
DR   InterPro; IPR011032; GroES-like_sf.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020843; PKS_ER.
DR   PANTHER; PTHR42813:SF3; GLUTATHIONE-INDEPENDENT FORMALDEHYDE DEHYDROGENASE; 1.
DR   PANTHER; PTHR42813; ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE; 1.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   SMART; SM00829; PKS_ER; 1.
DR   SUPFAM; SSF50129; GroES-like; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR   PROSITE; PS00059; ADH_ZINC; 1.
PE   3: Inferred from homology;
KW   Metal-binding {ECO:0000256|RuleBase:RU361277};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Zinc {ECO:0000256|RuleBase:RU361277}.
FT   DOMAIN          7..375
FT                   /note="Enoyl reductase (ER)"
FT                   /evidence="ECO:0000259|SMART:SM00829"
SQ   SEQUENCE   377 AA;  40087 MW;  64EE5F8C4F61A72B CRC64;
     MKAVVYQEPY SVAINEVDRP TIQHPNDVIV RITSTAICGS DLHMYEGRTA AEPGIVFGHE
     NMGIIEETGS GVAGLNRGDR VVMPFNVACG FCKNCMAGNT GFCLTVNPGF AGGAYGYVAM
     GPYTGGQAEY LRVPFADFNC LKLPADGSHE ADFVLLADIF PTGYHGCELA QVSPGESVVV
     YGAGPVGLMA AYSALLRGAA RVFCVDRVPE RLAKAEEIGA IPIDFSAGDP VEMIKEQTGG
     EGTDKGVDAV GYQAQVGDGS KEEPAIVLNS LIETVRPTGR LGVPGLYVPS DPGGPSEEAK
     KGMLLVSVGR MFEKGQVIGT GQCNVKRYNR QLRDMIIAGR AKPSFVVSHE LSLSEAPDAY
     RKFDQRIEGY TKVVLHP
//