ID   A0A1H3JUI5_9CLOT        Unreviewed;       227 AA.
AC   A0A1H3JUI5;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   24-JAN-2024, entry version 17.
DE   SubName: Full=Pyruvate formate lyase activating enzyme {ECO:0000313|EMBL:SDY43617.1};
GN   ORFNames=SAMN05192546_102117 {ECO:0000313|EMBL:SDY43617.1};
OS   Tindallia californiensis.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae; Tindallia.
OX   NCBI_TaxID=159292 {ECO:0000313|EMBL:SDY43617.1, ECO:0000313|Proteomes:UP000199230};
RN   [1] {ECO:0000313|EMBL:SDY43617.1, ECO:0000313|Proteomes:UP000199230}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APO {ECO:0000313|EMBL:SDY43617.1,
RC   ECO:0000313|Proteomes:UP000199230};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR   EMBL; FNPV01000002; SDY43617.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1H3JUI5; -.
DR   STRING; 159292.SAMN05192546_102117; -.
DR   Proteomes; UP000199230; Unassembled WGS sequence.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd01335; Radical_SAM; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR012840; NrdG2.
DR   InterPro; IPR034457; Organic_radical-activating.
DR   InterPro; IPR007197; rSAM.
DR   NCBIfam; TIGR02495; NrdG2; 1.
DR   PANTHER; PTHR30352:SF13; GLYCYL-RADICAL ENZYME ACTIVATING ENZYME YJJW-RELATED; 1.
DR   PANTHER; PTHR30352; PYRUVATE FORMATE-LYASE-ACTIVATING ENZYME; 1.
DR   Pfam; PF13353; Fer4_12; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01094; Uncharacterised_Radical_SAM_Su; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   4: Predicted;
KW   4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Lyase {ECO:0000313|EMBL:SDY43617.1};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Pyruvate {ECO:0000313|EMBL:SDY43617.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000199230};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT   DOMAIN          13..227
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000259|PROSITE:PS51918"
SQ   SEQUENCE   227 AA;  25953 MW;  8086363AEA105D0B CRC64;
     MRIGGIQKTS LIDYPGHLST VIFLRGCNFR CSYCHNAELL SIEGVQIERQ KMMDELITRR
     SFIDSVVISG GECTLSEDLR GFIQEIKSIG YGVKLDTNGT KPDIIEPLIK EGLIDYIAMD
     IKAPPEKYDQ IAGVCVDLDA INKSIRVIKN QMKNYEFRTT VSPEILTETE VLKIADWISD
     AKKYVLQNFR DNENVLCGRK KLTSYPAIRM TYLKEQLEPF FEEVILR
//