ID A0A1H3K5T5_9PSEU Unreviewed; 2331 AA.
AC A0A1H3K5T5;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE SubName: Full=PfaB family protein {ECO:0000313|EMBL:SDY46968.1};
GN ORFNames=SAMN05421504_105648 {ECO:0000313|EMBL:SDY46968.1};
OS Amycolatopsis xylanica.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Amycolatopsis.
OX NCBI_TaxID=589385 {ECO:0000313|EMBL:SDY46968.1, ECO:0000313|Proteomes:UP000199515};
RN [1] {ECO:0000313|EMBL:SDY46968.1, ECO:0000313|Proteomes:UP000199515}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CPCC 202699 {ECO:0000313|EMBL:SDY46968.1,
RC ECO:0000313|Proteomes:UP000199515};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Antibiotic biosynthesis. {ECO:0000256|ARBA:ARBA00004792}.
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005194}.
CC -!- SIMILARITY: Belongs to the thioester dehydratase family. FabA
CC subfamily. {ECO:0000256|ARBA:ARBA00006714}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP
CC synthases family. {ECO:0000256|RuleBase:RU003694}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FNON01000005; SDY46968.1; -; Genomic_DNA.
DR STRING; 589385.SAMN05421504_105648; -.
DR OrthoDB; 9778690at2; -.
DR UniPathway; UPA00094; -.
DR Proteomes; UP000199515; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0008693; F:(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity; IEA:InterPro.
DR GO; GO:0008659; F:(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0016746; F:acyltransferase activity; IEA:InterPro.
DR GO; GO:0034017; F:trans-2-decenoyl-acyl-carrier-protein isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd01287; FabA; 2.
DR CDD; cd00833; PKS; 1.
DR Gene3D; 3.30.70.3290; -; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR Gene3D; 3.10.129.10; Hotdog Thioesterase; 4.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 1.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR010083; FabA.
DR InterPro; IPR013114; FabA_FabZ.
DR InterPro; IPR029069; HotDog_dom_sf.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43074; OMEGA-3 POLYUNSATURATED FATTY ACID SYNTHASE PFAB-RELATED; 1.
DR PANTHER; PTHR43074:SF1; PKS_AT DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF07977; FabA; 3.
DR Pfam; PF00109; ketoacyl-synt; 2.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00825; PKS_KS; 1.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 1.
DR SUPFAM; SSF54637; Thioesterase/thiol ester dehydrase-isomerase; 4.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS52004; KS3_2; 1.
PE 3: Inferred from homology;
KW Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW Fatty acid biosynthesis {ECO:0000256|ARBA:ARBA00023160};
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00023160};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023160};
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000199515};
KW Transferase {ECO:0000256|RuleBase:RU003694}.
FT DOMAIN 3..415
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
SQ SEQUENCE 2331 AA; 250271 MW; 88147A8D96049D5B CRC64;
MRFEPIAIVG RGAVLPGALG PDAFWANLAH GRTSLGPAPE GRWRVPRERM MTTPDAPEDG
TWSDIGGYVR GFEFDPAGLA IDVRGLDPQV EWVLHGVRAA LREAGQEGPA PRAGLVLGNL
SFPTAGMAAY AEQVWRGAPR SDARNRFMSG LPAHLAAQAL GLGAGAFSLD AACASSLYAI
KLACDRLHDR GADLMVAGAV NCADDLFIHV GFSALSALSR TGQSRPFHRG ADGLVPAEGA
AFVALMRLSD VTDQDVFGVI RGVGLSNDGR GAGFLVPAEE GQQRAMRLAY EMAGIAPETV
SLLECHATGT PVGDAVEVRS AAKIFADHKG LAVGSAKSNV GHLITAAGVA GLLKVIGAMR
AGVRPATLHA DSPIDALDGT PLRLLQEAEP WDGPRRAAIS AFGFGGNNAH LVVDNVDLCG
AVDPLPPKRS EPVAIVAIGA RVGNGENSHD LRKALFSGAG DSARRETVDV KLTGLRVPPL
DLKRTLAQQL LVLEAAREAV EGLELSRERT MVLVGMGCDP EVARYGARWR AEDGDEFTPV
LTSAGVVGTM PNIVANRLNS HLDLAGPSFT VSAEEASGLV ALEIAARAIG AGEADAAVVG
AVDLSHEPVH RQALKDLGLD REPGDAAIVL VLKRLSDVDK SDVIALLDDV TSESDDLDFD
PGELFGNPHA AKGLLAVATA ALALRHRAIP RAGAPADPAF DVCAAEVAVK PLAGKEKRVL
VKACTSRPWV AEHSAKLRVY SGADKRGVIQ ALEAGRESDT GPARLVLLGD EEPAKHWLTH
GGPKPEGVAF RETPLTGEIG FVFTGGISAY PGMGRELLLA FPDLIEEIEA RSGSVREAAA
WAFDGPDSAP RTVLDQIWGT ALLSQVHAAI SRDLLGLEPS AALGYSAGES NALVALGVWT
DMRALIAEMN ASGMFTREVA GEFAAIRRAW ARAGIAGTRW KNYLVGIEPD LARKALDGEV
AAHLIAINSP DSCLIGGDVP ACERVLARLD PSYTLPIPYE LAVHTPELGE VRGQWWRLHN
RPATPPDGVR FYSCATTGSY LPTAERAADA LTAQAVGTMD FAGTVERAWD DGVRIFIEHG
PKTLCTDWIR RTLGDREHLA IPLDAQDGRS VRQLSRVAAE LVAAGVSVDS QALFDRLAAS
LPADREPDRV VTLPAHLPEI KVPETTMARA PHLPSVLSIV DTKGPLFDRK QLEQLAVGKI
SELFGELFAA QDGYARQTRM PSPPMLLADR VIDIDAVAGS MGTGTIRTET DVEPGSWYLD
STGRMPPGIL VEAGQADLLL ISWLGADLLN RGERVYRLLG CELTFHGSPP KPGEKLEYEI
HIDDHAELGG VRLFFFHYDC HVDGELRLSV RKGQAGFFTD QELAASAGVI WDPGREEPPA
GQLDPPAITG APRRYDADAI RAFAEGRPAD CFGKAWDITR SHSRTPRIDE GDLLFLREVT
DFEPAGGPWR RGYLRAETPI TPDDWFFEGH FKNDPCMPGT LMFQGCLQAM AFYLAACGFT
LGNDAWRFEP VPGAAYDLRC RGQVTPASKN LAYEVFVTEL SAGPNPTVFA DVLCTVDGVK
AFHAKRVGLR LVPDWPLEQW RDRLLAETEP ETGLVAEVDG VRFGYESLLS CAWGKPSGAF
GELYSRFDGH RRVARLPGPP YHFMTRITET TGKLGGMEVG SSVVADYEVP DRVWYFDENK
GTMPLAVLME VALQPCGWLA SFVGCAVEIA DDLLFRNLDG EGIVSGYITP ETKVIRTHAT
LRDISVTGTM IIVSFAVECF ADDTTVFTMD TVFGFFPKSA FEDQQGLRAS DAERAAIAAP
PGPVTDLTTR PAKYCAGPLR LAGPMLLMLD RVTGYDPAGG EAGLGRLTAE KDVDAGEWFF
KAHFFQDPVQ PGSLGIEAMY QLLQFYLIER GAGAGMTAPR FEVLTGQRPL VWKYRGQVVP
AHGVIGTELE ITEFGGNHAR AKAWLWVDGK RIYSAVDLGV RVVEGEPAGE EVLDVRADTW
LGDHRPTWTV PALPMMSVVD RLAPAEDAFV LEDIELRRWI PATGPIRLKS EKDGEKSTVL
AWRDATTPAL SRFEPVATAT IGTPGSRPQT LAPLANVETV TGFYASGSLF HGPAFQYLKS
LRRGSNGASG VLDASLGSVP RGRLNQGLLD AATHVIPHDS LWQWVSSIPS GQVAYPCRIS
RFEQFEPLPD SGEIEVEARF AGLDGRFPII DLQLIRAGRV LVAMRLVEVL MPTGSFGTAA
PEHRRAFLRD RTYVDGLGLS RTKAGSTTLT RSDVEQCDWL VGTVAQVYGL PPAARGRDHL
AQIAVRDHVA RQARVHPSAV DLSEDLLVAH VAGTPYRVEL EQRPEAVTVR S
//
