ID A0A1H3PNF0_9PROT Unreviewed; 486 AA.
AC A0A1H3PNF0;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=SAMN05421754_10449 {ECO:0000313|EMBL:SDZ02470.1};
OS Nitrosomonas sp. Nm58.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
OC Nitrosomonadaceae; Nitrosomonas.
OX NCBI_TaxID=200126 {ECO:0000313|EMBL:SDZ02470.1, ECO:0000313|Proteomes:UP000199646};
RN [1] {ECO:0000313|Proteomes:UP000199646}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nm58 {ECO:0000313|Proteomes:UP000199646};
RA Varghese N., Submissions S.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; FNPQ01000044; SDZ02470.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H3PNF0; -.
DR STRING; 200126.SAMN05421754_10449; -.
DR Proteomes; UP000199646; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000199646};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 6..133
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 145..454
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 486 AA; 54982 MW; 30399705E8EF8CA7 CRC64;
MTQQELERYL WGAATALRGT IDAGDYKQYI FPLLFFKRIS DVYDEEFSNA LAESDGDLEY
AAFAENHHFQ IPEGAHWNNV REKTVNIGTA LQEAMRAIEQ ANPNALFGIF GDASWTNKDR
LSDAILTNLI EHFSQHKLNV SNVPDDQLGN AYEYLIKEFA DDSGHTAAEF YTNRTVVKLM
TLIMDPQPGE SVYDPTCGSG GLLLNCALHL KDEGKEYRTL KLYGQEVNLL TSAIARMNMF
MHGIEEFSIV RGNTLTHPAF LEDDQLKTFN VILANPPYSI KAWDRKAFES DPYGRNLWGT
PPQGCADYAF QQHIQKSLDK KNGRSITLWP HGILFRDTEA DMRRKMIEQD LVECVIGLGP
NLFYNSPMEA CLLITKTNKE ASRKGKVLII NAVSEVRQDK NIAFLEPQHI ERIYAAYKAF
QDQNGFCKVV GIDQVLSHKA SLNMSLYVSN VNSSDTKISL NEALQNWMQS SAELKKSMDE
LFRVIG
//
