ID A0A1H4R6H3_9MICC Unreviewed; 480 AA.
AC A0A1H4R6H3;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex {ECO:0000256|RuleBase:RU003423};
DE EC=2.3.1.- {ECO:0000256|RuleBase:RU003423};
GN ORFNames=SAMN04489745_2472 {ECO:0000313|EMBL:SEC27492.1};
OS Arthrobacter woluwensis.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Micrococcaceae;
OC Arthrobacter.
OX NCBI_TaxID=156980 {ECO:0000313|EMBL:SEC27492.1, ECO:0000313|Proteomes:UP000182652};
RN [1] {ECO:0000313|EMBL:SEC27492.1, ECO:0000313|Proteomes:UP000182652}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 10495 {ECO:0000313|EMBL:SEC27492.1,
RC ECO:0000313|Proteomes:UP000182652};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=(R)-lipoate; Xref=ChEBI:CHEBI:83088;
CC Evidence={ECO:0000256|ARBA:ARBA00001938,
CC ECO:0000256|RuleBase:RU003423};
CC -!- SIMILARITY: Belongs to the 2-oxoacid dehydrogenase family.
CC {ECO:0000256|ARBA:ARBA00007317, ECO:0000256|RuleBase:RU003423}.
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DR EMBL; FNSN01000003; SEC27492.1; -; Genomic_DNA.
DR RefSeq; WP_066215638.1; NZ_FNSN01000003.1.
DR AlphaFoldDB; A0A1H4R6H3; -.
DR STRING; 156980.SAMN04489745_2472; -.
DR Proteomes; UP000182652; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR CDD; cd06849; lipoyl_domain; 1.
DR Gene3D; 2.40.50.100; -; 1.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 4.10.320.10; E3-binding domain; 1.
DR InterPro; IPR003016; 2-oxoA_DH_lipoyl-BS.
DR InterPro; IPR001078; 2-oxoacid_DH_actylTfrase.
DR InterPro; IPR000089; Biotin_lipoyl.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR036625; E3-bd_dom_sf.
DR InterPro; IPR004167; PSBD.
DR InterPro; IPR011053; Single_hybrid_motif.
DR PANTHER; PTHR43178; DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX; 1.
DR PANTHER; PTHR43178:SF5; LIPOAMIDE ACYLTRANSFERASE COMPONENT OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX, MITOCHONDRIAL; 1.
DR Pfam; PF00198; 2-oxoacid_dh; 1.
DR Pfam; PF00364; Biotin_lipoyl; 1.
DR Pfam; PF02817; E3_binding; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
DR SUPFAM; SSF47005; Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex; 1.
DR SUPFAM; SSF51230; Single hybrid motif; 1.
DR PROSITE; PS50968; BIOTINYL_LIPOYL; 1.
DR PROSITE; PS00189; LIPOYL; 1.
DR PROSITE; PS51826; PSBD; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|RuleBase:RU003423};
KW Lipoyl {ECO:0000256|ARBA:ARBA00022823, ECO:0000256|RuleBase:RU003423};
KW Pyruvate {ECO:0000313|EMBL:SEC27492.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000182652};
KW Transferase {ECO:0000256|RuleBase:RU003423, ECO:0000313|EMBL:SEC27492.1}.
FT DOMAIN 2..77
FT /note="Lipoyl-binding"
FT /evidence="ECO:0000259|PROSITE:PS50968"
FT DOMAIN 157..194
FT /note="Peripheral subunit-binding (PSBD)"
FT /evidence="ECO:0000259|PROSITE:PS51826"
FT REGION 104..151
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 222..252
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 480 AA; 49285 MW; 7957359D019954CB CRC64;
MSREFLLPDL GEGLTEAELV RWLVAVGDSV TIDQPVAEVE TAKAVVEVPS PFAGTVAVLH
GAEGETLDVG KPLISIDEGG ASAAPVLETA SDYRTEERAG SGNVLIGYGT SGSTDGGRSR
RRKTAAPAGL ASAGETPRIS PAPAQRDSVD GAEAVRVVSP LVRKLARDLG VALHALDGSG
PDGLILRRDV EQASAGSPAA SAAATAGANA VGPATATAGY AAGQDAPKGA GADEPTDHST
DPSTGLPIAA RTPLRGMRRT IAQAMVRSRT EIPEATVWVD VDATDLVELR AGLKRRDPEN
APSLLAFIAR FVLAGLAKYP KLNTRIETAE DGGQEIVSFD GVNLGFAAQT DRGLVVPSIR
AAHRLSARGL DGELKRLTGV ARDGKASPAD LSGSTFTLNN YGVFGVDGSA AIINHPEAAI
LGVGRIIDRP WVVDGALAVR KVAELSLVFD HRVCDGGTAG GFLRYVADAI ESPGTVLADL
//
