ID A0A1H5JDQ7_9ACTN Unreviewed; 551 AA.
AC A0A1H5JDQ7;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE RecName: Full=Neutral metalloproteinase {ECO:0000256|RuleBase:RU366073};
DE EC=3.4.24.- {ECO:0000256|RuleBase:RU366073};
GN ORFNames=SAMN05216489_06885 {ECO:0000313|EMBL:SEE50683.1};
OS Streptomyces sp. 3213.3.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1855348 {ECO:0000313|EMBL:SEE50683.1, ECO:0000313|Proteomes:UP000198754};
RN [1] {ECO:0000313|EMBL:SEE50683.1, ECO:0000313|Proteomes:UP000198754}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3213 {ECO:0000313|EMBL:SEE50683.1,
RC ECO:0000313|Proteomes:UP000198754};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Extracellular zinc metalloprotease.
CC {ECO:0000256|RuleBase:RU366073}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|RuleBase:RU366073};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|RuleBase:RU366073}.
CC -!- SIMILARITY: Belongs to the peptidase M4 family.
CC {ECO:0000256|ARBA:ARBA00009388, ECO:0000256|RuleBase:RU366073}.
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DR EMBL; FNTK01000001; SEE50683.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H5JDQ7; -.
DR STRING; 1855348.SAMN05216489_06885; -.
DR Proteomes; UP000198754; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09597; M4_TLP; 1.
DR Gene3D; 3.10.170.10; -; 1.
DR Gene3D; 3.10.450.40; -; 1.
DR Gene3D; 3.10.450.490; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR InterPro; IPR011096; FTP_domain.
DR InterPro; IPR023612; Peptidase_M4.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR InterPro; IPR001570; Peptidase_M4_C_domain.
DR InterPro; IPR013856; Peptidase_M4_domain.
DR PANTHER; PTHR33794; BACILLOLYSIN; 1.
DR PANTHER; PTHR33794:SF1; BACILLOLYSIN; 1.
DR Pfam; PF07504; FTP; 1.
DR Pfam; PF01447; Peptidase_M4; 1.
DR Pfam; PF02868; Peptidase_M4_C; 1.
DR PRINTS; PR00730; THERMOLYSIN.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU366073};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049,
KW ECO:0000256|RuleBase:RU366073};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU366073};
KW Reference proteome {ECO:0000313|Proteomes:UP000198754};
KW Secreted {ECO:0000256|RuleBase:RU366073};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|RuleBase:RU366073};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU366073}.
FT SIGNAL 1..35
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT CHAIN 36..551
FT /note="Neutral metalloproteinase"
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT /id="PRO_5023144174"
FT DOMAIN 82..128
FT /note="FTP"
FT /evidence="ECO:0000259|Pfam:PF07504"
FT DOMAIN 223..372
FT /note="Peptidase M4"
FT /evidence="ECO:0000259|Pfam:PF01447"
FT DOMAIN 376..550
FT /note="Peptidase M4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02868"
FT ACT_SITE 366
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
FT ACT_SITE 453
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
SQ SEQUENCE 551 AA; 56672 MW; 4EC25339B13BD2F1 CRC64;
MTPLYARHKR TTLAIATAVA AGALLATGVT TGASAQPATG KAAPLAAAPQ LLSNAARTAL
VKEKQADAAE TATQIGLGTK EKLVVKDVVK DVDGTVHTRY ERTYAGLPVL GGDLVVHTSP
AGKTEGVTKA HSAAIKVATV TPKITVAKAE KQALTAAKAA GSDKTAADGA RKVIWAGGAT
PVLAYETVVG GLQDDGTPNQ LHVITDAATG KKLFEYQGIE NATGTGKTLY SGTVSLTTTL
SGTTYSLTDA SRGSHKTYNL KHGTSGTGTL FTNTTNTWGT GAASSSSTDV TAAADAAYGA
QETWDFYKDT FGRSGIKNNG VGAYSRVHYG SSYVNAFWDD SCFCMTYGDG SGNTHPLTSL
DVAGHEMSHG VTANTAGLNY SGESGGLNEA TSDIFGTGVE FYANNSSDPG DYLIGEKIDI
NGDGSPLRYM DQPSKDGGSA NYWSSSVGNL DVHYSSGVAN HFFYLLSEGS GAKTINGVSY
NSPTYNGSTV TGIGRAKALQ IWYKALTTYF TSTTKYAGAR TGTLSAAAAL YGSGSTEYNA
VAAAWSAVNV S
//
