ID A0A1H5RCQ9_9PSEU Unreviewed; 1085 AA.
AC A0A1H5RCQ9;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=SAMN05421837_109310 {ECO:0000313|EMBL:SEF36153.1};
OS Amycolatopsis pretoriensis.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Amycolatopsis.
OX NCBI_TaxID=218821 {ECO:0000313|EMBL:SEF36153.1, ECO:0000313|Proteomes:UP000198878};
RN [1] {ECO:0000313|EMBL:SEF36153.1, ECO:0000313|Proteomes:UP000198878}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44654 {ECO:0000313|EMBL:SEF36153.1,
RC ECO:0000313|Proteomes:UP000198878};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; FNUJ01000009; SEF36153.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H5RCQ9; -.
DR STRING; 218821.SAMN05421837_109310; -.
DR Proteomes; UP000198878; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF59; DNA HELICASE; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 39..352
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 355..655
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 734..756
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 734..755
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 60..67
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1085 AA; 116934 MW; 0599E70A2D01F2A7 CRC64;
MPVRHPGERA WFHRWVNARR TGRADARLVR PPLTDAPTFT WDDGARRVLS APGGFRRVLG
GPGTGKTSLL ASAATRRIAE GADPESVLVL TTSRKAADAL RADITRRLTF DPESARPLPR
TVREPLVRTV HSYAYSLLRL EAMAEELPPP RLLAGAEQDV VVRELLAGDL DEEAGYWPEQ
LRPALMVPGF AEELRDLLMR AAERGLGPED LAELGRRRGR EEWIAAGQFW AQYEEVTQLQ
GAGGNALGVA SSPALDAAEL VTSALLALED DDELRMRERT RVRHLFVDDA HHLDPLQTSL
VRVIGHTAAE FVVAGDPDQN VFSFRGADAS LFADADPDGS RTVTLTTAHR LAPAVRLAVA
KIGATLPGAS QHRKILPPAG ASGGNVRVRV MPTPAAEASW IADQLRRAHL VDGVPWSEIA
VLVRSPARTF LVLQRALRAA GVPIGSATEE LPLARQPAVR PLLAVLKLAP APDLLDVDLA
EMLLSSALGG ADPLALRRLR RGLRRLELAG GGQRSSDELL VEALRGGDIL AGLADAESEP
VRRVGGLLRV THQAVARGDG VEQVLWQLWT ESRLQEKLLK QVERGGSLGA QADRDLDAVV
ALFDAAGRYV DRLPRASVAS FADYLGAQQI AGDTLAPAAV PSDGVSLLTA HAAAGREWTV
VAVAGVQEGA WPDLRLRGSV LGVERLKDLM SGVDDDAVSQ TAPILAEERR LFYLAMSRAK
QTLLVTAVSG EDEQPSRFLD DLEENGADDG GLDSRMKPPG RSLVLAELVG ELREVVCDDK
ADPVRRRRAA KQLARLADAK VPGAHPSTWY GLLPASTDEP VHRPGDLVRI SPSTVEILTK
CPLRWMIERH GGSDPAQLAA VTGTLVHGLA QAVASGRTEA ELQAALDEAW VRVDAGAPWF
SRRERRRVEQ MLQNFVTWLE RSRAELKAAG VEQDIEVELP AGGEDEVRVL LRGRVDRVEL
DAEGRPVIVD IKTGKVPVSG KDAEEHPQLA AYQLAVLLGA IKGSNEPGGA RLVYVAKSNK
QTGSTERSQP PLDEVGGKQW LELVRDAAAS AAGPDYQAQE NPDCDRCPAR GCCPLRPEGR
QVPGP
//
