ID   A0A1H6E298_9ACTN        Unreviewed;       586 AA.
AC   A0A1H6E298;
DT   18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT   18-JAN-2017, sequence version 1.
DT   27-MAR-2024, entry version 23.
DE   SubName: Full=3-oxosteroid 1-dehydrogenase {ECO:0000313|EMBL:SEG91687.1};
GN   ORFNames=SAMN05444920_107329 {ECO:0000313|EMBL:SEG91687.1};
OS   Nonomuraea solani.
OC   Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC   Streptosporangiaceae; Nonomuraea.
OX   NCBI_TaxID=1144553 {ECO:0000313|EMBL:SEG91687.1, ECO:0000313|Proteomes:UP000236732};
RN   [1] {ECO:0000313|EMBL:SEG91687.1, ECO:0000313|Proteomes:UP000236732}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CGMCC 4.7037 {ECO:0000313|EMBL:SEG91687.1,
RC   ECO:0000313|Proteomes:UP000236732};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974};
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DR   EMBL; FNVT01000007; SEG91687.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1H6E298; -.
DR   OrthoDB; 9813348at2; -.
DR   Proteomes; UP000236732; Unassembled WGS sequence.
DR   GO; GO:0033765; F:steroid dehydrogenase activity, acting on the CH-CH group of donors; IEA:UniProt.
DR   GO; GO:0008202; P:steroid metabolic process; IEA:UniProt.
DR   Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR   InterPro; IPR003953; FAD-binding_2.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR027477; Succ_DH/fumarate_Rdtase_cat_sf.
DR   InterPro; IPR006311; TAT_signal.
DR   PANTHER; PTHR43400:SF7; FAD_BINDING_2 DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR43400; FUMARATE REDUCTASE; 1.
DR   Pfam; PF00890; FAD_binding_2; 1.
DR   PRINTS; PR00411; PNDRDTASEI.
DR   SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR   SUPFAM; SSF56425; Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain; 1.
DR   PROSITE; PS51318; TAT; 1.
PE   4: Predicted;
KW   Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Reference proteome {ECO:0000313|Proteomes:UP000236732}.
FT   DOMAIN          46..568
FT                   /note="FAD-dependent oxidoreductase 2 FAD binding"
FT                   /evidence="ECO:0000259|Pfam:PF00890"
SQ   SEQUENCE   586 AA;  61775 MW;  D87074481264A766 CRC64;
     MRPVVPAPEG LTAEGRSRRT LLIAGGILVT GAALGQNPAY AQADYDVVVV GSGAAGMTAA
     LTAAKRGLSV VVLEKAPTYG GTAARSGAGI WIPNNEVILA AGVPDTPAKA AAYLARVVGG
     TVPIERQQAF LAAGPAMISF VMRNSPLRFR FMEGYADYYP EYPGGMPNGR SIEPDQFDGN
     RLGAELARLN APYMAVPAGM VVFSADYKWL TLSAVHPKGI EVSAAALLRG TEAALRGEKP
     LTMGQALAGA LRAGLMAAGV PVWLNTPLLD LHVEAGRVAG VVTSAGVVRA RRGVIVGSGG
     FERSAAMRAE FQRQPIGTAW TLGARENTGD GIEAGERAGA ALELMDDAWW GPVIPLPGEP
     YFCLAERTLP GGLIVNGNGA RFVNEGAPYS DVVHVMYDRN TPSAPHIPAW LIVDQQYRNR
     YLFKDIAPLL PFPDSWYASG AVHKAWTLEQ LASRIGVPPA ALRNTVTRFN GFAQTGRDLD
     FQRGDSAYDR YYADPYVKPN PCLAPLWLPP FYAFKIQPGD LGTKGGLRTD ARSRALRADG
     SVIPGLYAAG NASGAVMGHS YAGSGSTIGP AMTFGYIAAN HIADNP
//