ID A0A1H6EM78_9ACTN Unreviewed; 837 AA.
AC A0A1H6EM78;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=SAMN05444920_111234 {ECO:0000313|EMBL:SEG98201.1};
OS Nonomuraea solani.
OC Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC Streptosporangiaceae; Nonomuraea.
OX NCBI_TaxID=1144553 {ECO:0000313|EMBL:SEG98201.1, ECO:0000313|Proteomes:UP000236732};
RN [1] {ECO:0000313|EMBL:SEG98201.1, ECO:0000313|Proteomes:UP000236732}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 4.7037 {ECO:0000313|EMBL:SEG98201.1,
RC ECO:0000313|Proteomes:UP000236732};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|PROSITE-ProRule:PRU10059, ECO:0000256|RuleBase:RU361166}.
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DR EMBL; FNVT01000011; SEG98201.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H6EM78; -.
DR OrthoDB; 9808897at2; -.
DR Proteomes; UP000236732; Unassembled WGS sequence.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030247; F:polysaccharide binding; IEA:UniProtKB-UniRule.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR CDD; cd02850; E_set_Cellulase_N; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 2.60.40.290; -; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001919; CBD2.
DR InterPro; IPR008965; CBM2/CBM3_carb-bd_dom_sf.
DR InterPro; IPR012291; CBM2_carb-bd_dom_sf.
DR InterPro; IPR004197; Cellulase_Ig-like.
DR InterPro; IPR003305; CenC_carb-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR Pfam; PF00553; CBM_2; 1.
DR Pfam; PF02018; CBM_4_9; 1.
DR Pfam; PF02927; CelD_N; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SMART; SM00637; CBD_II; 1.
DR SUPFAM; SSF49384; Carbohydrate-binding domain; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS51173; CBM2; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000236732};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 26..837
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5009029651"
FT DOMAIN 723..837
FT /note="CBM2"
FT /evidence="ECO:0000259|PROSITE:PS51173"
FT ACT_SITE 658
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 706
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 715
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 837 AA; 90923 MW; FF176D542F709EFC CRC64;
MTRCRAALLA LFTMVALFPA TAAQAADEGP EQIVNGTFDT GTAPWWGTAN VTLAVTDGRL
CADIPGGTTN PWDVIIGQDN IPLVEGDTYA YRFFAAATPA KVGRALIQLP VDPWTQYLSA
APEMSVSGNS YSYTFTAPVS LPNAQVAFQL GGSAEPWRFC MDDVSLKGGA EPEVYEPDTG
PRVRVNMVGY LPAGPKKATV VTTATEAVDW ELKNAAKETV ASGRTTPRGV DASSGQNVHT
LDFGSYRKAG KEYTLTADGE TSRPFDIAAD LYDQLPVDAL KFYYTQRSGI EILDGLRPGY
GRPAGHVGVA PNQGDVSVPC QPGVCDYSLN VKGGWYDAGD HGKYVVNGGI SVFQLMATYE
RDKATFKDRQ LNIPESGNGT PDILDEARWE QEFLLSMQVP DGKPYAGMAH HKIHDQNWTG
LPLLPHLDPQ LRELHPTSTA ATLNLAATAA QAARLYAPYD GKFALRNLEA ARKAWTAAKA
NPERYADPTD GTGGGAYNDP DVSDEFYWAA AELYITTGEK EFRDFVLTSP HHTGDIWRER
GFDWGNTAQL GRLDLATVPN ALPDRARVRQ SVIEGAEKYL GIQRAHPYGL SYNPPDFDWG
SNNLVLNNLV VMATAYDLTG QEKYRAGVRE GMDYILGRNA LNQSYVTGYG EVASKNQHSR
WYARQLDPAL PNPPRGTLAG GPNSGLQDPL AQRLLRGCKP QFCYIDHIES WATNELTINW
NSPLAWVSAF LADRGERSDC RVRYSAHGAG SFAAQVAIEN TGRHTVDAWA LRWSFLGGQR
VRQGWGAALS QAGPVVTAKG QGRIKPGRTV TFGFTATNPG GPNPAPEVFW LNGARCK
//
